Activation of pertussis toxin-sensitive G-proteins in membranes of SH-SY5Y human neuroblastoma cells and bovine transducin by ethanol
| Autor: | Hans Rommelspacher, Jan F. Klinker, Henning Damm, Birgit Lichtenberg-Kraag |
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| Rok vydání: | 1996 |
| Předmět: |
Time Factors
G protein GTPase Guanosine triphosphate Biology Pertussis toxin Sensitivity and Specificity Neuroblastoma chemistry.chemical_compound GTP-Binding Proteins Retinal Rod Photoreceptor Cells Heterotrimeric G protein Tumor Cells Cultured Animals Humans Transducin Virulence Factors Bordetella Ethanol Hydrolysis General Neuroscience Cell Membrane Central Nervous System Depressants Enkephalin Leucine-2-Alanine Membrane Pertussis Toxin chemistry Biochemistry Receptors Opioid Cattle Guanosine Triphosphate Signal transduction |
| Zdroj: | Neuroscience Letters. 213:25-28 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(96)12816-0 |
| Popis: | Effects of ethanol on signal transduction in neuronal membranes are supposed to occur by the interaction with heterotrimeric guanine nucleotide-binding proteins (G-proteins). Several substances affect signal transduction by activation of G-proteins directly independent of receptors. We show that similar to those substances, ethanol stimulates high-affinity guanosine triphosphate (GTP)-hydrolysis in SH-SY5Y membranes at concentrations of 50 mM and higher in a pertussis toxin-sensitive manner. Compared with ethanol at a concentration of 170 mM, other alcohols were without or with respect to methanol with a slight effect on high-affinity GTP-hydrolysis in SH-SY5Y membranes. Ethanol also stimulates the GTPase of the purified G-protein transducin. The findings suggest that ethanol affects signal transduction in neuronal membranes by direct activation of pertussis toxin-sensitive G-proteins. |
| Databáze: | OpenAIRE |
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