Transcriptional comparison of human induced and primary midbrain dopaminergic neurons
| Autor: | Fang Fang, Benjamin Angulo, Renee A. Reijo Pera, Ninuo Xia, Pengbo Zhang, James G. Taylor, Zhengyuan Wang, Rosaria Chiang, Megan Rothstein |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Pluripotent Stem Cells
0301 basic medicine Neurogenesis Cellular differentiation Induced Pluripotent Stem Cells LIM-Homeodomain Proteins Bioinformatics Article Nestin 03 medical and health sciences Mesencephalon hemic and lymphatic diseases Humans Medicine RNA Messenger Induced pluripotent stem cell Cells Cultured Embryonic Stem Cells Oligonucleotide Array Sequence Analysis Otx Transcription Factors Multidisciplinary Sequence Analysis RNA business.industry Dopaminergic Neurons Gene Expression Profiling Dopaminergic nutritional and metabolic diseases Cell Differentiation Parkinson Disease Embryonic stem cell Gene expression profiling 030104 developmental biology nervous system Hepatocyte Nuclear Factor 3-beta Metabolome Neuron maturation Transcriptome business Neuroscience Transcription Factors |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep20270 |
| Popis: | Generation of induced dopaminergic (iDA) neurons may provide a significant step forward towards cell replacement therapy for Parkinson’s disease (PD). To study and compare transcriptional programs of induced cells versus primary DA neurons is a preliminary step towards characterizing human iDA neurons. We have optimized a protocol to efficiently generate iDA neurons from human pluripotent stem cells (hPSCs). We then sequenced the transcriptomes of iDA neurons derived from 6 different hPSC lines and compared them to that of primary midbrain (mDA) neurons. We identified a small subset of genes with altered expression in derived iDA neurons from patients with Parkinson’s Disease (PD). We also observed that iDA neurons differ significantly from primary mDA neurons in global gene expression, especially in genes related to neuron maturation level. Results suggest iDA neurons from patient iPSCs could be useful for basic and translational studies, including in vitro modeling of PD. However, further refinement of methods of induction and maturation of neurons may better recapitulate full development of mDA neurons from hPSCs. |
| Databáze: | OpenAIRE |
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