Heparin-binding keratinocyte growth factor is a candidate stromal-to-epithelial-cell andromedin

Autor: Yoshitatsu Fukabori, Guochen Yan, Wallace L. McKeehan, Fen Wang, Stathis Nikolaropoulos
Rok vydání: 1992
Předmět:
Male
Fibroblast Growth Factor 7
Neoplasms
Hormone-Dependent
Stromal cell
medicine.medical_treatment
Cellular differentiation
Molecular Sequence Data
Biology
Fibroblast growth factor
Epithelium
Paracrine signalling
chemistry.chemical_compound
Endocrinology
Tumor Cells
Cultured
medicine
Animals
Testosterone
Northern blot
Receptor
Fibroblast Growth Factor
Type 2
Growth Substances
Fibroblast
Molecular Biology
Cells
Cultured
Connective Tissue Cells
Base Sequence
Growth factor
Prostate
Prostatic Neoplasms
Receptor Protein-Tyrosine Kinases
Dihydrotestosterone
Epithelial Cells
General Medicine
Fibroblasts
Protein-Tyrosine Kinases
Receptors
Fibroblast Growth Factor
Rats
Cell biology
Fibroblast Growth Factors
medicine.anatomical_structure
Gene Expression Regulation
chemistry
Connective Tissue
Cancer research
Keratinocyte growth factor
Fibroblast Growth Factor 10
Cell Division
Zdroj: Molecular Endocrinology. 6:2123-2128
ISSN: 1944-9917
0888-8809
Popis: The growth of isolated epithelial and stromal cells from both androgen-dependent normal rat prostate and an androgen-responsive model rat prostate tumor is androgen-independent. When added to co-cultures of epithelial and stromal cells separated by a semipermeable membrane, androgen stimulated epithelial cell growth without an effect on stromal cell growth. Northern blot and nuclease protection analysis of mRNA revealed that stromal cells specifically expressed an androgen-sensitive secreted member of the heparin-binding fibroblast growth factor family [keratinocyte growth factor (KGF)/fibroblast growth factor-7]. KGF was mitogenic for epithelial cells, but not for stromal cells. Epithelial cells expressed specifically a splice variant of the bek receptor gene that specifically binds KGF. Expression of the bek receptor gene in stromal cells was undetectable by Northern blot and nuclease protection analyses. The results suggest that stromal cell-derived KGF has the properties of an andromedin, which mediates the indirect control of epithelial cell proliferation by androgen through a directional stromal-to-epithelial cell paracrine mechanism.
Databáze: OpenAIRE
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