Heparin-binding keratinocyte growth factor is a candidate stromal-to-epithelial-cell andromedin
Autor: | Yoshitatsu Fukabori, Guochen Yan, Wallace L. McKeehan, Fen Wang, Stathis Nikolaropoulos |
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Rok vydání: | 1992 |
Předmět: |
Male
Fibroblast Growth Factor 7 Neoplasms Hormone-Dependent Stromal cell medicine.medical_treatment Cellular differentiation Molecular Sequence Data Biology Fibroblast growth factor Epithelium Paracrine signalling chemistry.chemical_compound Endocrinology Tumor Cells Cultured medicine Animals Testosterone Northern blot Receptor Fibroblast Growth Factor Type 2 Growth Substances Fibroblast Molecular Biology Cells Cultured Connective Tissue Cells Base Sequence Growth factor Prostate Prostatic Neoplasms Receptor Protein-Tyrosine Kinases Dihydrotestosterone Epithelial Cells General Medicine Fibroblasts Protein-Tyrosine Kinases Receptors Fibroblast Growth Factor Rats Cell biology Fibroblast Growth Factors medicine.anatomical_structure Gene Expression Regulation chemistry Connective Tissue Cancer research Keratinocyte growth factor Fibroblast Growth Factor 10 Cell Division |
Zdroj: | Molecular Endocrinology. 6:2123-2128 |
ISSN: | 1944-9917 0888-8809 |
Popis: | The growth of isolated epithelial and stromal cells from both androgen-dependent normal rat prostate and an androgen-responsive model rat prostate tumor is androgen-independent. When added to co-cultures of epithelial and stromal cells separated by a semipermeable membrane, androgen stimulated epithelial cell growth without an effect on stromal cell growth. Northern blot and nuclease protection analysis of mRNA revealed that stromal cells specifically expressed an androgen-sensitive secreted member of the heparin-binding fibroblast growth factor family [keratinocyte growth factor (KGF)/fibroblast growth factor-7]. KGF was mitogenic for epithelial cells, but not for stromal cells. Epithelial cells expressed specifically a splice variant of the bek receptor gene that specifically binds KGF. Expression of the bek receptor gene in stromal cells was undetectable by Northern blot and nuclease protection analyses. The results suggest that stromal cell-derived KGF has the properties of an andromedin, which mediates the indirect control of epithelial cell proliferation by androgen through a directional stromal-to-epithelial cell paracrine mechanism. |
Databáze: | OpenAIRE |
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