Cell surface expression of channel catfish leukocyte immune-type receptors (IpLITRs) and recruitment of both Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 and SHP-2
| Autor: | Deborah N. Burshtyn, Jacqueline Mewes, Chelsea L. Davidson, James L. Stafford, Benjamin C. Montgomery |
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| Rok vydání: | 2009 |
| Předmět: |
animal structures
Molecular Sequence Data Immunology Protein Tyrosine Phosphatase Non-Receptor Type 11 Protein tyrosine phosphatase Biology Transfection src Homology Domains Mice chemistry.chemical_compound Cell Line Tumor Leukocytes Animals Humans Amino Acid Sequence Receptors Immunologic Tyrosine Zebrafish Innate immune system Base Sequence Protein Tyrosine Phosphatase Non-Receptor Type 6 Cell Membrane Tyrosine phosphorylation Molecular biology Cell biology Ictaluridae chemistry embryonic structures Immunoglobulin superfamily Phosphorylation Signal transduction Sequence Alignment HeLa Cells Developmental Biology Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Developmental & Comparative Immunology. 33:570-582 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2008.10.006 |
| Popis: | Channel catfish leukocyte immune-type receptors (IpLITRs) are immunoglobulin superfamily (IgSF) members believed to play a role in the control and coordination of cellular immune responses in teleost. Putative stimulatory and inhibitory IpLITRs are co-expressed by different types of catfish immune cells (e.g. NK cells, T cells, B cells, and macrophages) but their signaling potential has not been determined. Following cationic polymer-mediated transfections into human cell lines we examined the surface expression, tyrosine phosphorylation, and phosphatase recruitment potential of two types of putative inhibitory IpLITRs using 'chimeric' expression constructs and an epitope-tagged 'native' IpLITR. We also cloned and expressed the teleost Src homology 2 domain-containing protein tyrosine phosphatases (SHP)-1 and SHP-2 and examined their expression in adult tissues and developing zebrafish embryos. Co-immunoprecipitation experiments support the inhibitory signaling potential of distinct IpLITR-types that bound both SHP-1 and SHP-2 following the phosphorylation of tyrosine residues within their cytoplasmic tail (CYT) regions. Phosphatase recruitment by IpLITRs represents an important first step in understanding their influence on immune cell effector functions and suggests that certain inhibitory signaling pathways are conserved among vertebrates. |
| Databáze: | OpenAIRE |
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