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Additional file 1: Figure S1. Characterization of the functionalized CaP-MA nanoparticles. Synthetic route (A) and 1H NMR (B) and FTIR (C) spectrum of the conjugated mannitol-alendronate (MA-AL); Size distribution and polydispersity index (PDI) of CaP (D), CaP-MA-5 (E), CaP-MA-20 (F) and CaP-MA-40 (G); Morphology of CaP and different CaP-MA nanoparticles (H); Evaluation the abilities of different nanoparticles to protect DNA (I), (+) represented incubation with DNase I and (-) represented incubation without DNase I, mean ± SD, n=3. ★The intensity was too low to be determined. Figure S2. The biocompatibility of CaP and CaP-MA-5/20/40 nanoparticles. Cell viability of CaP nanoparticles, CaP-MA nanoparticles (A), and MA-AL (B) measured by the MTT assay. The mixture of fresh blood and nanoparticles after 1 hour incubation (C), Lip-2000 (a), CaP-MA-40 (b), CaP-MA-20 (c), CaP-MA-5 (d), CaP (e), saline (f), Triton-X (g). Hemolytic rates of Lip-2000, CaP and CaP-MA-5/20/40 nanoparticles (D). Data are shown as mean ± SD (n=3). * P |
