Estimating Diversity of Black Flies in theSimulium ignescensandSimulium tunjaComplexes in Colombia: Chromosomal Rearrangements as the Core of Integrative Taxonomy
| Autor: | Nubia E. Matta, Paulina Muñoz de Hoyos, Marta L. Bueno, Peter H. Adler, Fredy A. Colorado-Garzón, Luis Fernando García |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine Species complex Biodiversity Morphological variation Colombia 010603 evolutionary biology 01 natural sciences Translocation Genetic Evolution Molecular 03 medical and health sciences Genetics Animals DNA Barcoding Taxonomic Simuliidae Selection Genetic Simulium Simulium ignescens Molecular Biology Psilopelmia Phylogeny Genetics (clinical) Polytene Chromosomes Polytene chromosome biology Ecology fungi Sequence Analysis DNA biology.organism_classification Phylogeography Genes Mitochondrial 030104 developmental biology Evolutionary biology Female Taxonomy (biology) Biotechnology |
| Zdroj: | Journal of Heredity. 108:12-24 |
| ISSN: | 1465-7333 0022-1503 |
| DOI: | 10.1093/jhered/esw063 |
| Popis: | Black flies (Diptera: Simuliidae) are distributed throughout the world, with more than 2200 formally described species. The family is renowned for its high frequency of cryptic species, offering an opportunity for integrative taxonomy, based on morphological, chromosomal, and molecular approaches. The biodiversity within Simulium (Psilopelmia) ignescens and S. (Psilopelmia) tunja in Colombia was estimated from the larval stage; 10 morphoforms were recognized based on 7 structural characters. This remarkable morphological variation was evaluated through 23 markers on the polytene chromosomes. We established 1 new cytoform in each nominal species. The congruence of the morphological and chromosomal assignments was evaluated using the mitochondrial marker Cytochrome Oxidase subunit I (COI) for each morphoform. The molecular data supported the chromosomal recognition of cytoforms (i.e., cryptic species). We also established the suitability of the COI marker for linking the pupal stage with each cytoform. Our results reveal the presence of hidden biodiversity in S. ignescens and S. tunja and demonstrate the power of polytene chromosomes as a tool for evaluating simuliid diversity, while illustrating the importance of integrated analyses in modern taxonomy. |
| Databáze: | OpenAIRE |
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