Task-shifting of CD4 T cell count monitoring by the touchscreen-based Muse™ Auto CD4/CD4% single-platform system for CD4 T cell numeration: Implication for decentralization in resource-constrained settings
| Autor: | Marcel Mbeko Simaleko, Gérard Grésenguet, Christian Diamant Mossoro-Kpinde, Laurent Bélec, André Kouabosso, Jean De Dieu Longo, Ralph-Sydney Mboumba Bouassa |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male 0301 basic medicine Coefficient of variation Immunology Resource constrained HIV Infections 03 medical and health sciences 0302 clinical medicine Monitoring Immunologic parasitic diseases Statistics Absolute bias Humans Immunology and Allergy 030212 general & internal medicine Laboratory technicians Mathematics Automation Laboratory Observer Variation Absolute number Cd4 t cell Politics Reproducibility of Results Middle Aged Flow Cytometry CD4 Lymphocyte Count 030104 developmental biology Absolute count HIV-1 Female Task shifting |
| Zdroj: | Journal of Immunological Methods. 455:95-98 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2018.01.008 |
| Popis: | Background The accuracy of CD4 T cell monitoring by the recently developed flow cytometry-based CD4 T cell counting Muse™ Auto CD4/CD4% Assay analyzer (EMD Millipore Corporation, Merck Life Sciences, KGaA, Darmstadt, Germany) was evaluated in trained lay providers against laboratory technicians. Methods After 2 days of training on the Muse™ Auto CD4/CD4% analyzer, EDTA-blood samples from 6 HIV-positive and 4 HIV-negative individuals were used for CD4 T cell counting in triplicate in parallel by 12 trained lay providers as compared to 10 lab technicians. Results Mean number of CD4 T cells in absolute number was 829 ± 380 cells/μl by lay providers and 794 ± 409 cells/μl by technicians (P > 0.05); and in percentage 36.2 ± 14.8%CD4 by lay providers and 36.1 ± 15.0%CD4 by laboratory technician (P > 0.05). The unweighted linear regression and Passing-Bablok regression analyses on CD4 T cell results expressed in absolute count revealed moderate correlation between CD4 T cell counts obtained by lay providers and lab technicians. The mean absolute bias measured by Bland–Altman analysis between CD4 T cell/μl obtained by lay providers and lab technicians was −3.41 cells/μl. Intra-assay coefficient of variance (CV) of Muse™ Auto CD4/CD4% in absolute number was 10.1% by lay providers and 8.5% by lab technicians (P > 0.05), and in percentage 5.5% by lay providers and 4.4% by lab technicians (P > 0.05). The inter-assay CV of Muse™ Auto CD4/CD4% in absolute number was 13.4% by lay providers and 10.3% by lab technicians (P > 0.05), and in percentage 7.8% by lay providers and 6.9% by lab technicians (P > 0.05). Conclusions The study demonstrates the feasibility of CD4 T cell counting using the alternative flow cytometer Muse™ Auto CD4/CD4% analyzer by trained lay providers and therefore the practical possibility of decentralization CD4 T cell counting to health community centers. |
| Databáze: | OpenAIRE |
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