Ultra-sparse Connectivity within the Lateral Hypothalamus
| Autor: | Mahesh M. Karnani, Denis Burdakov |
|---|---|
| Přispěvatelé: | Saints-Pères Paris Institute for the Neurosciences |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Lateral hypothalamus Synaptic connectivity Patch clamp Orexin MCH Gamma oscillation [SDV]Life Sciences [q-bio] Action Potentials Mice Transgenic Neocortex Sensory system Biology Optogenetics Inhibitory postsynaptic potential Whole-Cell Recordings General Biochemistry Genetics and Molecular Biology Membrane Potentials Mice Neural activity 03 medical and health sciences 0302 clinical medicine Report Connectome medicine Animals 030304 developmental biology Melanins Neurons Orexins 0303 health sciences Hypothalamic Hormones Brain Waves Mice Inbred C57BL Pituitary Hormones 030104 developmental biology medicine.anatomical_structure Hypothalamic Area Lateral Excitatory postsynaptic potential General Agricultural and Biological Sciences Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Current Biology-CB Current Biology-CB, Elsevier, 2020, 30, pp.4063-4070.e2. ⟨10.1016/j.cub.2020.07.061⟩ Current Biology, 30 (20) Current Biology |
| ISSN: | 0960-9822 1879-0445 |
| DOI: | 10.3929/ethz-b-000447189 |
| Popis: | Summary The lateral hypothalamic area (LH) is a vital controller of arousal, feeding, and metabolism [1, 2], which integrates external and internal sensory information. Whereas sensory and whole-body output properties of LH cell populations have received much interest, their intrinsic synaptic organization has remained largely unstudied. Local inhibitory and excitatory connections could help integrate and filter sensory information and mutually inhibitory connections [3] could allow coordinating activity between LH cell types, some of which have mutually exclusive behavioral effects, such as LH VGLUT2 and VGAT neurons [4, 5, 6, 7] and orexin- (ORX) and melanin-concentrating hormone (MCH) neurons [8, 9, 10]. However, classical Golgi staining studies did not find interneurons with locally ramifying axons in the LH [11, 12], and nearby subthalamic and thalamic areas lack local synaptic connectivity [13, 14]. Studies with optogenetic circuit mapping within the LH have demonstrated only a minority of connections when a large pool of presynaptic neurons was activated [15, 16, 17, 18, 19]. Because multiple patch clamp has not been used to study LH connectivity, aside from a limited dataset of MCH neurons where no connections were discovered [15], we used quadruple whole-cell recordings to screen connectivity within the LH with standard methodology we previously used in the neocortex [20, 21, 22]. Finding a lack of local connectivity, we used optogenetic circuit mapping to study the strength of LH optogenetic responses and network oscillations, which were consistent with ultra-sparse intrinsic connectivity within the LH. These results suggest that input from other brain structures is decisive for selecting active populations in the LH. Graphical Abstract Highlights • LH neurons within It is unknown how neural activity is coordinated among lateral hypothalamic area (LH) neurons, which generate fundamental behavioral actions such as attack and evasion. Burdakov and Karnani show there is near-zero local connectivity in the LH, suggesting that incoming synaptic input is integrated primarily within individual neurons. |
| Databáze: | OpenAIRE |
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