CX3CR1+ cells facilitate the activation of CD4 T cells in the colonic lamina propria during antigen-driven colitis
| Autor: | Christian U. Riedel, Paul Walther, Jan Hendrik Niess, C Manta, V Rossini, K Radulovic, D Zhurina |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Adoptive cell transfer Ovalbumin Immunology CX3C Chemokine Receptor 1 T-Cell Antigen Receptor Specificity chemical and pharmacologic phenomena Biology Lymphocyte Activation Green fluorescent protein Interleukin 21 Mice Immune system Antigen Intestinal mucosa Antigens CD medicine Escherichia coli Immunology and Allergy Mesenteric lymph nodes Animals Mesentery Antigens Intestinal Mucosa 610 Medicine & health Mice Knockout Phagocytes hemic and immune systems Dendritic Cells Colitis Molecular biology Disease Models Animal medicine.anatomical_structure Phenotype biology.protein Female Receptors Chemokine Lymph Nodes Integrin alpha Chains |
| DOI: | 10.7892/boris.53048 |
| Popis: | Dendritic cells (DCs) and macrophages populate the intestinal lamina propria to initiate immune responses required for the maintenance of intestinal homeostasis. To investigate whether CX3CR1(+) phagocytes communicate with CD4 T cells during the development of transfer colitis, we established an antigen-driven colitis model induced by the adoptive transfer of DsRed OT-II cells in CX3CR1(GFP/+) × RAG(-/-) recipients challenged with Escherichia coli expressing ovalbumin (OVA) fused to a cyan fluorescent protein (CFP). After colonization of CX3CR1(GFP/+) × RAG(-/-) animals with red fluorescent E. coli pCherry-OVA, colonic CX3CR1(+) cells but not CD103(+) DCs phagocytosed E. coli pCherry-OVA. Degraded bacterial-derived antigens are transported by CD103(+) DCs to mesenteric lymph nodes (MLNs), where CD103(+) DCs prime naive T cells. In RAG(-/-) recipients reconstituted with OT II cells and gavaged with OVA-expressing E. coli, colonic CX3CR1(+) phagocytes are in close contact with CD4 T cells and presented bacterial-derived antigens to CD4 T cells to activate and expand effector T cells. |
| Databáze: | OpenAIRE |
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