Triosephosphate Isomerase- and Glyceraldehyde-3-Phosphate Dehydrogenase-Reactive Autoantibodies in the Cerebrospinal Fluid of Patients with Multiple Sclerosis
| Autor: | Johanna Kölln, Neal Hermanowicz, Reng-Rong Da, Michael J. Olek, Yufen Qin, Yiping Zhang, Stanley van den Noort, Edzard Spillner, Lutz G.W. Hilgenberg, Hui-Min Ren, Martin A. Smith |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Multiple Sclerosis Immunology Autoantigens Triosephosphate isomerase Cerebrospinal fluid medicine Humans Immunology and Allergy Single-chain variable fragment In patient Glyceraldehyde 3-phosphate dehydrogenase Autoantibodies Neurons B-Lymphocytes Clinically isolated syndrome biology business.industry Multiple sclerosis Autoantibody Glyceraldehyde-3-Phosphate Dehydrogenases Middle Aged medicine.disease Axons biology.protein Female Nervous System Diseases business Triose-Phosphate Isomerase |
| Zdroj: | The Journal of Immunology. 177:5652-5658 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Our previous results revealed that Igs in lesions and single chain variable fragment Abs (scFv-Abs) generated from clonal B cells in the cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) bind to axons in MS brains. To study the axonal Ags involved in MS, we identified the glycolytic enzymes, triosephosphate isomerase (TPI) and GAPDH, using Igs from the CSF and scFv-Abs generated from clonal B cells in the CSF and in lesions from MS patients. Elevated levels of CSF-Abs to TPI were observed in patients with MS (46%), clinically isolated syndrome (CIS) suggestive of MS (40%), other inflammatory neurological diseases (OIND; 29%), and other noninflammatory neurological diseases (ONIND; 31%). Levels of GAPDH-reactive Abs were elevated in MS patients (60%), in patients with CIS (10%), OIND (14%), and ONIND (8%). The coexistence of both autoantibodies was detected in 10 MS patients (29%), and 1 CIS patient (3%), but not in patients with OIND/ONIND. Two scFv-Abs generated from the CSF and from lesions of a MS brain showed immunoreactivity to TPI and GAPDH, respectively. The findings suggest that TPI and GAPDH may be candidate Ags for an autoimmune response to neurons and axons in MS. |
| Databáze: | OpenAIRE |
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