Urine can speed up the re-epithelialization process of prostatic urethra wounds by promoting the proliferation and migration of prostate epithelial cells
| Autor: | Guangheng Luo, Ying Cao, Zhaolin Sun, Ye Tian, Xiushu Yang, Lixin Wang |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Urology medicine.medical_treatment Prostatic Hyperplasia 030232 urology & nephrology H&E stain Urine 030204 cardiovascular system & hematology 03 medical and health sciences Dogs 0302 clinical medicine Ureter Re-Epithelialization Urethra Cell Movement Prostate Prostatic urethra medicine Animals Humans Intraoperative Complications Cells Cultured Cell Proliferation Wound Healing Benign prostatic hyperplasia Urology - Original Paper Prostatectomy business.industry Transurethral Resection of Prostate Epithelial Cells Histology medicine.anatomical_structure Nephrology Transforming Growth Factors Histopathology business |
| Zdroj: | International Urology and Nephrology |
| ISSN: | 1573-2584 0301-1623 |
| DOI: | 10.1007/s11255-018-2019-2 |
| Popis: | Objectives The present study aimed to investigate the influence of urine on re-epithelialization in canine prostatic urethra after prostatectomy and explore possible causes. Method We established two groups of prostatic canine models. The first group contained urine that canines underwent the surgery by two-micron laser resection of the prostate-tangerine technique (TmLRP-TT), and no transurethral catheter was required. The second group was without urine that canines accepted the surgery by TmLRP-TT add ureter skin ostomy urine bypass. Histopathology of re-epithelialization of repair in trauma in canine prostatic urethra was observed by hematoxylin and eosin (HE) staining, and immunochemistry was used to determine the expression of transforming growth factor-β1 (TGF-β1). Human prostate epithelial line (BPH-1) cells were cultured with or without urine and the abilities of proliferation and migration were tested by CCK-8 and transwell assays, respectively. Results The histology displayed that there was distinct proliferation of prostatic cell under the wound after 3 days, re-epithelialization began after 9 days, and finished after 28 days at urine group. The TGF-β1 like-IR in prostatic epithelium cells and fibroblast cells under the wound at urine group were strikingly increased as compared with the cells at no urine group after 3, 9, and 11 days, respectively (p |
| Databáze: | OpenAIRE |
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