Metformin ameliorates insulitis in STZ-induced diabetic mice
| Autor: | Jia-Wen Yu, Yu-Long Tao, Ya-Ping Deng, Guo-Fei Ren, Guo-Jun Jiang, Jian Cai, Xue Han, Yuannan Sun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system diseases medicine.medical_treatment lcsh:Medicine 030209 endocrinology & metabolism General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine STZ Internal medicine Diabetes mellitus DM medicine Pathological Pharmacology business.industry General Neuroscience Insulin lcsh:R digestive oral and skin physiology Anti-inflammatory effect nutritional and metabolic diseases General Medicine medicine.disease Metformin Diabetes and Endocrinology 030104 developmental biology medicine.anatomical_structure Endocrinology Panreatic islets Immunohistochemistry General Agricultural and Biological Sciences Pancreas business Insulitis medicine.drug |
| Zdroj: | PeerJ PeerJ, Vol 5, p e3155 (2017) |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.3155 |
| Popis: | Background & AimsMetformin is currently the most widely used first-line hypoglycemic agent for diabetes mellitus. Besides glucose-lowering action, there is increasingly interest in the potential anti-inflammatory action of this drug. In the present study, we investigated the actions of metformin on experimental insulitis using STZ-induced diabetic mice.MethodsMice with acute diabetes induced by STZ were administered metformin by gavage. Changes of blood glucose and body weight, and the daily amount of food and water intake were measured. Pancreatic tissues were collected for histologic analyses. Pathological assessment and immunohistochemistry analysis were used to determine the effect of metformin on insulitis. Inflammatory cytokines in the pancreas and insulin levels were measured through ELISA analysis.ResultsMetformin significantly reduced blood glucose levels and improved aberrant water intake behavior in experimental diabetic mice. No significant differences were observed in terms of body weight and food intake behavior in metformin-treated animals. In the STZ-induced model of diabetes, we found the appearance of pronounced insulitis. However, metformin administration reduced the severity of insulitis assessed by blind pathological scoring. In addition, metformin treatment improved insulin levels in experimental diabetic mice. ELISA assay revealed decreased levels of inflammatory response marker IL-1βand TNF-αin the pancreatic tissues following metformin treatment.ConclusionMetformin attenuated insulitis in the STZ-induced mice model of diabetes. This islet-protective effect might be partly correlated with the anti-inflammatory action of metformin. |
| Databáze: | OpenAIRE |
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