Analysis of two-dimensional electrophoretic patterns of proteins obtained from the sera of normal and tumorbearing nude rats
| Autor: | Shin Furuya, Yoshifumi Tanimoto, Toshio Imai, Ikunosuke Sakurabayashi, Michio Itoh, Yoshida Motoko |
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| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Chromatography Spots Clinical Biochemistry Biology Blood Protein Electrophoresis Biochemistry Blood proteins Molecular biology Neoplasm Proteins Rats Analytical Chemistry Rats Nude Electrophoresis chemistry Inbred strain Animals Electrophoresis Gel Two-Dimensional Glycoprotein Polyacrylamide gel electrophoresis Human cancer Glycoproteins |
| Zdroj: | Electrophoresis. 12:80-83 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | To classify two-dimensional electrophoresis (2-DE) patterns of normal and abnormal samples, a new parameter, named shift value, is introduced. The shift value is defined by the product of three indices; differences in density, differences in area, and the Euclid distance between peaks of matched glycoprotein spots in the 2-DE patterns. Shift values obtained from the differences between the 2-DE patterns of tumor-bearing and normal sera (control) were always found to be larger than those obtained from two control patterns. The shift value obtained from glycoprotein spots could be more effectively used to distinguish normal and abnormal patterns than that obtained from simple proteins. In our earlier attempts to compare serum proteins of normal and tumor patients, we could not discriminate differences caused by genetic background. In order to circumvent this difficulty, we employed an inbred strain of nude rats into which various types of human cancer had been implanted. Reliability of the analysis is enhanced by using a polyacrylamide gel backed with a silanized glass support and an automatic 2-DE apparatus. |
| Databáze: | OpenAIRE |
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