A highly sensitive method to quantify triazolam and its metabolites with liquid chromatography--mass spectrometry
| Autor: | Tadao Mimura, Kimiyoshi Kohta, Toshiaki Takahashi, Tomoyuki Fujita, Naoto Senda, Mitsuru Nakayama, Kenichi Shizukuishi |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Triazolam Clinical Biochemistry Urine Mass spectrometry Biochemistry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Liquid chromatography–mass spectrometry Drug Discovery medicine Humans Molecular Biology Biotransformation Chromatography High Pressure Liquid Pharmacology Chemical ionization Chromatography General Medicine Hydrogen-Ion Concentration Solvent chemistry Methanol Ammonium acetate medicine.drug |
| Zdroj: | Biomedical chromatography : BMC. 9(1) |
| ISSN: | 0269-3879 |
| Popis: | We established a highly sensitive method to determine triazolam and its major metabolites, alpha-hydroxytriazolam and 4-hydroxytriazolam, in human plasma and urine with a liquid chromatography-mass spectrometry system which incorporates an atmospheric chemical ionization interface. A plasma sample and a urine sample after solvent extraction were injected into an ODS column of reversed phase with a mobile phase in a linear solvent gradient of initially 50 mM ammonium acetate (pH 4.0) 50%:methanol 50% and 15 min later methanol 100%; quantification limits of as low as 20 pg/mL at an SNR of 3 were obtained for each compound. With diazepam as an internal standard, recovery yields of 84, 81, and 77% were obtained for triazolam, alpha-hydroxytriazolam and 4-hydroxytriazolam, respectively. |
| Databáze: | OpenAIRE |
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