Cripto-1 expression in uveal melanoma: An immunohistochemical study
| Autor: | Subramanian Krishnakumar, Kandalam Mallikarjuna, Pushparaj Vaijayanthi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Uveal Neoplasms Cell type Pathology medicine.medical_specialty Melanocyte Eye GPI-Linked Proteins Cripto medicine.disease_cause Metastasis Immunoenzyme Techniques Cellular and Molecular Neuroscience Biomarkers Tumor Tumor Cells Cultured medicine Humans Neoplasm Invasiveness Melanoma Aged Membrane Glycoproteins Epidermal Growth Factor business.industry Liver Neoplasms Cancer Middle Aged medicine.disease eye diseases Sensory Systems Neoplasm Proteins Ophthalmology medicine.anatomical_structure Intercellular Signaling Peptides and Proteins Melanocytes Immunohistochemistry Female sense organs business Carcinogenesis Sclera hormones hormone substitutes and hormone antagonists |
| Zdroj: | Experimental Eye Research. 84:1060-1066 |
| ISSN: | 0014-4835 |
| Popis: | Human Cripto, the founder member of the epidermal growth factor-Cripto-FRL1-Cryptic (EGF-CFC) family, plays an important role during early embryonic development and in particular in carcinogenesis and the development of cancer metastases. Cripto-1 is over-expressed in most cancers, but is absent or only weakly expressed in normal cells. For this reason, Cripto-1 could be of potential value in the targeted treatment. There is no information on the expression of Cripto-1 in human uveal melanoma. Cripto-1 reactivity was evaluated by immunohistochemistry on 36 archival uveal melanomas using the polyclonal antibody to Cripto-1. The tumors were divided in to 2 groups. There were 18 uveal melanomas with no intrascleral or extrascleral extension and 18 uveal melanomas with intrascleral/extrascleral extension/liver metastasis. Cripto-1 reactivity was correlated with tumor aggressiveness and cell type. Furthermore, we studied the immunolocalization of Cripto-1 in 4 uveal melanoma cell lines OCM-1, OCM-8, and 92-1, and OMM-1 and in 2 primary uveal melanocyte cultures. Cripto-1 was expressed in both the non-invasive and aggressive uveal melanomas. Cripto-1 was positive in the 4 uveal melanoma cell lines and absent in the primary uveal melanocyte cultures. Retinal tissue did not express Cripto-1. The results suggest that Cripto-1 is expressed in uveal melanoma, negative in the non-neoplastic ocular tissue and point to its use as a target for therapy. |
| Databáze: | OpenAIRE |
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