Zebrafish (Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences
| Autor: | Gro-Ingunn Hemre, Andreas Nordgreen, Knut G Berdal, Lene E. Johannessen, Nini H. Sissener, Ernst M. Hevrøy, Christer R. Wiik-Nielsen |
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| Rok vydání: | 2009 |
| Předmět: |
Male
DNA Plant Danio Medicine (miscellaneous) Models Biological Zea mays Superoxide dismutase chemistry.chemical_compound Transcription (biology) Animals Food science Animal Husbandry Gene Zebrafish DNA Primers Sex Characteristics Deoxyribonucleases Nutrition and Dietetics Base Sequence biology Pesticide Residues food and beverages biology.organism_classification Animal Feed Hsp70 Biochemistry chemistry RNA Plant Toxicity biology.protein Female Soybeans DNA |
| Zdroj: | British Journal of Nutrition. 103:3-15 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s0007114509991401 |
| Popis: | A 20-d zebrafish (Danio rerio) feeding trial, in which a near doubling of fish weight was achieved, was conducted with GM feed ingredients to evaluate feed intake, growth, stress response and uptake of dietary DNA. A partial aim of the study was to assess zebrafish as a model organism in GM safety assessments. Roundup Ready®soya (RRS®), YieldGard®Bt maize (MON810) and their non-modified, maternal, near-isogenic lines were used in a 2 × 2 factorial design. Soya variety and maize variety were the main factors, both with two levels; non-GM and GM. Compared with fish fed non-GM maize, those fed GM maize exhibited significantly better growth, had lower mRNA transcription levels ofsuperoxide dismutase (SOD)-1and a tendency (non-significant) towards lower transcription ofheat shock protein 70in liver. Sex of the fish and soya variety had significant interaction effects on total RNA yield from the whole liver and transcription ofSOD-1, suggesting that some diet component affecting males and females differently was present in different levels in the GM and the non-GM soya used in the present study. Dietary DNA sequences were detected in all of the organs analysed, but not all of the samples. Soya and maizerubisco(non-transgenic, multicopy genes) were most frequently detected, while MON810 transgenic DNA fragments were detected in some samples and RRS®fragments were not detected. In conclusion, zebrafish shows promise as a model for this application. |
| Databáze: | OpenAIRE |
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