Hepatic CCR7lowCD62LlowCD45RClow allograft dendritic cells migrate to the splenic red pulp in immunologically unresponsive rats
| Autor: | Katsutaka Mori, Yasuo Yamaguchi, Feng-Shan Wang, Takashi Furuhashi, Kazutoshi Okabe, Shinichi Kihara, Hajime Ohshiro, Michio Ogawa, Shinichiro Uchino, Shinwa Yamada |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
White pulp Receptors CCR7 Pathology medicine.medical_specialty Receptors CCR5 Receptors Lymphocyte Homing Immunoglobulins C-C chemokine receptor type 7 Spleen Biology Mucoproteins Cell Movement medicine Animals Transplantation Homologous Blood Transfusion L-Selectin Antigen-presenting cell Rats Inbred Strains Dendritic Cells Dendritic cell Marginal zone Antigens Differentiation Liver Transplantation Rats medicine.anatomical_structure Splenic Red Pulp Models Animal Leukocyte Common Antigens Pulp (tooth) Receptors Chemokine Transplantation Tolerance Surgery |
| Zdroj: | Journal of Surgical Research. 124:29-37 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2004.08.030 |
| Popis: | Donor dendritic cells (DC) migrate into the recipient spleen after hepatic transplantation. Immunological unresponsiveness to rat hepatic allografts can be induced by prior donor-specific blood transfusion (DST). We investigated homing receptor phenotype and splenic distribution of donor DC after allografting and DST. Immunostaining revealed OX62+ cells in the splenic red pulp of animals receiving pre-transplant DST but only in the white pulp of untreated animals. Most OX62 cells were positive for OX76. There were two subsets of DC in the spleen, CD45RChighOX62+ and CD45RClowOX62+ cells. RT-PCR revealed that CD45RClowOX62+ cells expressed interleukin (IL)-10, while CD45RChighOX62+ cells expressed IL-2 and low levels of IL-10 mRNA. CD45RChighOX62+ cells strongly expressed CCR5 and CCR7, compared with weak expression in CD45RClowOX62+ cells. The Epstein-Barr virus-induced molecule 1 (EBI-1) ligand chemokine (ELC/MIP3beta) was expressed mainly within the splenic white pulp. Mucosal vascular addressin-cell adhesion molecule-1 (MAdCAM-1) was expressed in the marginal zone and white pulp, but expression of splenic MAdCAM-1 was down-regulated in DST-treated animals. L-selectin (CD62L), the ligand for MAdCAM-1, was strongly expressed on CD45RChighOX62+ cells but not on CD45RClowOX62+ cells. In conclusion, differential splenic migration of CCR5lowCCR7lowCD62Llow CD45RClow DC expressing Th2-type cytokines is associated with immunological unresponsiveness to rat hepatic allografts. |
| Databáze: | OpenAIRE |
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