UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds
| Autor: | Yakov Demurin, Waltraud Mair, Pavel V. Mazin, Sergei Garkusha, Alina Chernova, Rim F. Gubaev, D. V. Goryunov, Lyudmila Gorlova, Nikolai Anikanov, Anna Vanushkina, Elena Martynova, Zhanna Mukhina, Svetlana V. Goryunova, Philipp Khaytovich |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine cold resistance sunflower Rapeseed spring-type rapeseed High variability lcsh:QR1-502 rapeseed winter-type rapeseed 01 natural sciences Biochemistry Article Mass Spectrometry lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Food science triglycerides Molecular Biology Chromatography High Pressure Liquid Freezing tolerance Triglyceride ultraperformance liquid chromatography–mass spectrometry (UPLC–MS) Cold resistance Brassica rapa freezing tolerance Sunflower 030104 developmental biology Vegetable oil chemistry Seeds Helianthus Uplc ms ms 010606 plant biology & botany |
| Zdroj: | Biomolecules, Vol 9, Iss 1, p 9 (2018) Biomolecules Volume 9 Issue 1 |
| ISSN: | 2218-273X |
| Popis: | Sunflower and rapeseed are among the most important sources of vegetable oil for food and industry. The main components of vegetable oil are triglycerides (TAGs) (about 97%). Ultra- performance liquid chromatography coupled with mass spectrometry (UPLC&ndash MS) profiling of TAGs in sunflower and rapeseed has been performed and the TAG profiles obtained for these species have been compared. It has been identified that 34 TAGs are shared by sunflower and rapeseed. It was demonstrated that TAGs 52:2, 52:5, 52:6, 54:3 54:4, 54:7, 56:3, 56:4, and 56:5 had the highest variability levels between sunflower and rapeseed with the higher presence in rapeseed. TAGs 50:2, 52:3, 52:4, 54:5, and 54:6 also showed high variability, but were the most abundant in sunflower. Moreover, the differences in TAG composition between the winter-type and spring-type rapeseed have been revealed, which may be associated with freezing tolerance. It was shown that winter-type rapeseed seeds contain TAGs with a lower degree of saturation, while in spring-type rapeseed highly saturated lipids are the most abundant. These findings may give new insights into the cold resistance mechanisms in plants the understanding of which is especially important in terms of global climate changes. |
| Databáze: | OpenAIRE |
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