Granulocyte colony-stimulating factor enhances protection by anti-K1 capsular IgM antibody in murine Escherichia coli sepsis
| Autor: | Kees Kraaijeveld, Jan Verhoef, J. Kuenen, K. P. M. Van Kessel, L Tavares, Andy I. M. Hoepelman, Barry J. Benaissa-Trouw, Willem N. M. Hustinx |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Survival
Neutrophils medicine.medical_treatment Clinical Biochemistry Bacteremia Complement receptor Peritonitis Granulocyte Biochemistry Sepsis Mice Adjuvants Immunologic Phagocytosis Granulocyte Colony-Stimulating Factor medicine Animals Receptor Bacterial Capsules Escherichia coli Infections Antigens Bacterial Mice Inbred BALB C biology Tumor Necrosis Factor-alpha Polysaccharides Bacterial Receptors IgG General Medicine medicine.disease Antibodies Bacterial Granulocyte colony-stimulating factor Cytokine medicine.anatomical_structure Immunoglobulin M Fluorescent Antibody Technique Direct Luminescent Measurements Immunology biology.protein Female Tumor necrosis factor alpha Antibody Fluorescein-5-isothiocyanate |
| Zdroj: | European Journal of Clinical Investigation. 27:1044-1048 |
| ISSN: | 1365-2362 0014-2972 |
| Popis: | Combined prophylactic treatment with recombinant murine granulocyte colony-stimulating factor (G-CSF) and a suboptimal dose of anti-K1 capsular IgM monoclonal antibody (MAb) significantly enhanced survival in an experimental mouse Escherichia coli O7:K1 peritonitis model compared with untreated animals (67% vs. 11% survival; P < 0.001) and with either treatment alone (67 vs. 29% and 27% survival, respectively; P < 0.01), which suggests synergism between these agents. Enhanced survival by combined treatment was associated with increased neutrophil counts in blood and peritoneal lavage fluid, lower systemic and higher levels of local tumour necrosis factor (TNF) and lower bacterial counts in blood cultures. Mouse neutrophils treated with G-CSF but not infected with E. coli showed enhanced phagocytic and respiratory burst capacity, down-regulation of L-selectin receptors and enhanced expression of Fc RII-III receptors but not of complement receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text