Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants
| Autor: | Jason Z. Zhang, Hsien-Wei Yeh, Alexandra C. Walls, Basile I. M. Wicky, Kaitlin R. Sprouse, Laura A. VanBlargan, Rebecca Treger, Alfredo Quijano-Rubio, Minh N. Pham, John C. Kraft, Ian C. Haydon, Wei Yang, Michelle DeWitt, John E. Bowen, Cameron M. Chow, Lauren Carter, Rashmi Ravichandran, Mark H. Wener, Lance Stewart, David Veesler, Michael S. Diamond, Alexander L. Greninger, David M. Koelle, David Baker |
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| Rok vydání: | 2022 |
| Předmět: |
SARS-CoV-2
Prevention Biomedical Engineering COVID-19 Bioengineering Biosensing Techniques Pneumonia Applied Microbiology and Biotechnology Antibodies Spike Glycoprotein Coronavirus Vaccine Related Emerging Infectious Diseases Infectious Diseases Neutralization Tests Biodefense Pneumonia & Influenza Molecular Medicine Humans Viral Neutralizing Lung Biotechnology |
| Zdroj: | Nature biotechnology, vol 40, iss 9 |
| Popis: | We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays. |
| Databáze: | OpenAIRE |
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