Selection of genes repressed by cAMP that are induced by nutritional limitation inSaccharomyces cerevisiae
| Autor: | Michel Jacquet, Djamila Tadi, Emmanuelle Boy-Marcotte, Rukhsana Nilofer Hasan, Françoise Bussereau |
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| Přispěvatelé: | Université Paris-Sud - Paris 11 (UP11) |
| Rok vydání: | 1999 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Cell Saccharomyces cerevisiae Bioengineering Polymerase Chain Reaction Applied Microbiology and Biotechnology Biochemistry stress Transformation Genetic Gene Expression Regulation Fungal Cyclic AMP Genetics medicine Selection Genetic DNA Fungal Msn2p Gene ComputingMilieux_MISCELLANEOUS DNA Primers Gene Library Msn4 Double mutant biology STRE Trehalose RNA Fungal Sequence Analysis DNA Blotting Northern beta-Galactosidase biology.organism_classification Hedgehog signaling pathway Yeast Glucose medicine.anatomical_structure Lac Operon Metabolic enzymes cAMP-dependent protein kinase (cAPK) Glycogen Signal Transduction Biotechnology |
| Zdroj: | Yeast Yeast, Wiley, 1999, 15 (16), pp.1733-1745. ⟨10.1002/(SICI)1097-0061(199912)15:163.0.CO;2-L⟩ |
| ISSN: | 1097-0061 0749-503X |
| DOI: | 10.1002/(sici)1097-0061(199912)15:16<1733::aid-yea490>3.0.co;2-l |
| Popis: | DNA–lacZ fusion libraries of yeast Saccharomyces cerevisiae were used to select genes coordinately regulated by the Ras–cAMP–cAPK signalling pathway. Sixteen new genes (AGP1, APE2, APE3, FPS1, GUT2, MDH2, PLB2, PYK2, RNR3, SUR1, UGA1, YHR033w, YBR006w, YHR143w, YMR086w and YOR173w) were found to be repressed by cAMP. Most of these genes encode for metabolic enzymes and are induced by nutritional limitations. These common properties suggest a role of this pathway in the metabolic adjustment of the cell to nutritional variations. The induction of 10 of these genes is reduced in the msn2,msn4 double mutant, which emphasizes the role of the Msn2/4p transcriptional activators in mediating the Ras–cAMP–cAPK signalling pathway. The Msn2p/Msn4p-independent expression of the six other genes suggests the existence of other regulatory systems under the control of this pathway. Copyright © 1999 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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