Liquid‐based cytological and immunohistochemical study of nasopharyngeal swab from persons under investigation for SARS‐CoV‐2 infection
| Autor: | Josep Gumà, Karla B. Peña, Francesc Riu, David Parada, Carmen Guilarte |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty RT‐PCR Histology medicine.drug_class Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses Cytological Techniques swab Monoclonal antibody Virus SARS‐CoV‐2 nasopharynx Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine COVID‐19 Cytology viral transport medium medicine Humans Aged Aged 80 and over business.industry SARS-CoV-2 fungi COVID-19 Original Articles liquid‐based General Medicine Middle Aged Immunohistochemistry Nucleoprotein 030104 developmental biology Real-time polymerase chain reaction immunohistochemical 030220 oncology & carcinogenesis cytology Liquid based Female Original Article business |
| Zdroj: | Histopathology |
| ISSN: | 1365-2559 0309-0167 |
| DOI: | 10.1111/his.14257 |
| Popis: | INTRODUCTION: We describe cytologic and immunohistologic findings in virus transport medium on cases under investigation of SARS-CoV-2 infection. METHODS: Cytologic findings in cases under investigation of SARS-CoV-2 infection from one hundred consecutive nasopharyngeal swab were reviewed. Immunohistochemistry and SARSCoV-2 RT-PCR determination were performed to detect virus. RESULTS: No viral inclusions were noted in squamous cells obtained from virus transport medium. Immunohistochemical study with monoclonal antibody against SARS-CoV-2 viral nucleoprotein showed positivity in squamous cells. No positivity was present in others cellular components. CONCLUSIONS: SARS-CoV-2 predominantly localizes squamous cells in cytology samples of patients with RT-PCR positive determination of SARSCoV-2. The results of the current study support the notion that the nasopharyngeal region is the anatomical station that SARS-CoV-2 infects first, and the infection can lead to the migration of the virus into the lower airways. |
| Databáze: | OpenAIRE |
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