Intradermal DNA vaccination in ear pinnae is an efficient route to protect cats against rabies virus
| Autor: | Alvaro Aguilar-Setién, Rafael Hernández-González, Francisco Blanco-Favela, Emiliano Tesoro-Cruz, Rafael Calderón-Rodríguez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
rabies medicine.disease_cause Antibodies Viral Cat Diseases Group A Group B Mice 0302 clinical medicine [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Vaccines DNA Medicine 030212 general & internal medicine 0303 health sciences Mice Inbred BALB C CATS intranasal [SDV.BA]Life Sciences [q-bio]/Animal biology Antibody titer 3. Good health intradermal into ear pinna Titer [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDV.IMM]Life Sciences [q-bio]/Immunology Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Ear Auricle DNA vaccine Injections Intradermal Blotting Western [SDV.BC]Life Sciences [q-bio]/Cellular Biology Cell Line 03 medical and health sciences Neutralization Tests Animals Seroconversion 030304 developmental biology General Veterinary business.industry Rabies virus cats Immunization Passive Viral Vaccines [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology medicine.disease Virology [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics DNA Viral Rabies [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie business |
| Zdroj: | Veterinary Research Veterinary Research, BioMed Central, 2008, 39 (2), pp.1-11. ⟨10.1051/vetres:2007054⟩ |
| ISSN: | 0928-4249 1297-9716 |
| DOI: | 10.1051/vetres:2007054⟩ |
| Popis: | International audience; A DNA vaccine against rabies (pGQH) was administrated to cats in order to examine different administration routes. Four groups of three cats each were inoculated with pGQH as follows: group A, intramuscularly (IM), 100 $\mu$g; group B, intranasally (IN), 100 $\mu$g; group C, intradermally into ear pinnae (ID-EP), 100 $\mu$g, and group D, IM, 200 $\mu$L of phosphate buffer solution (PBS) alone (control group). Blood was drawn on days 0, 30, 60, 90, 120, 150, and 180. Groups A, B, and C received a booster on day 30. At day 200 all animals were challenged. A passive transfer of cat sera, as well as a viral challenge, was performed in mice. The results displayed that neutralizing antibody titers were higher in cats of group C (ID-EP) showing high early titers ($>$ 2 IU) and the highest titer was on day 120 ($>$ 14 IU). In group B (IN), two out of three cats seroconverted on day 30 ($>$ 0.5 IU), the third cat seroconverted until day 60 ($>$ 0.5 IU). In contrast, the lowest levels of neutralizing antibodies were detected in group A (IM). The control group showed no anti-rabies antibodies. Groups A (IM) and D (control) succumbed after lethal challenge. All animals from the ID-EP group (C) survived, only one individual from the IN (B) group died. Mice that received cat sera from ID-EP, IM, and IN groups survived and were protected (30/30 survivors). Mice groups that received pre-immunization sera from cats were not protected (0/30 survivors). This study demonstrates that pGQH immunization was successful when it was administrated ID-EP, and acceptable through the IN route. The IM route, however, was not effective in cats. For vaccination, the IN route seems attractive due to its accessibility for application, but it seems to activate seroconversion slowly. The best route to promote anti-rabies antibody titers was the ID-EP route. This practical and efficient route should be further studied. |
| Databáze: | OpenAIRE |
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