Emission-tunable probes using terbium(III)-doped self-activated luminescent hydroxyapatite for in vitro bioimaging
| Autor: | Seon Woo Kang, Il-Woo Lee, Caifeng Wang, Ki-Jae Jeong, Se-Joon Oh, Jaebeom Lee, Jieun Kang, Jeonghyo Kim, In Ho Han |
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| Rok vydání: | 2021 |
| Předmět: |
Lanthanide
Luminescence Materials science Biocompatibility chemistry.chemical_element Terbium 02 engineering and technology Conjugated system 010402 general chemistry Photochemistry Lanthanoid Series Elements 01 natural sciences Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Trisodium citrate Doping 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Durapatite chemistry Hydroxyapatites 0210 nano-technology |
| Zdroj: | Journal of Colloid and Interface Science. 581:21-30 |
| ISSN: | 0021-9797 |
| Popis: | Lanthanide ion (Ln3+)-doped nanoscale hydroxyapatites (nHAp) with tunable luminescence have attracted increasing attention due to their potential applications as useful biomedical tools (e.g., imaging and clinical therapy). In this study, we reported that doping Terbium (III) ions (Tb3+) in self-activated luminescent nHAp via a facile hydrothermal reaction, using trisodium citrate (Cit3−), generates unique emission-tunable probes known as Cit/Tb-nHAp. The morphology, crystal phase, and luminescence properties of these Cit/Tb-nHAp probes are studied in detail. Moreover, the results demonstrate that the luminescence of self-activated nHAp originates from the carbon dots trapped within the nHAp crystals, in which partial energy transfer occurs from carbon dots (CDs) to Tb3+. The color tunability is successfully achieved by regulating the addition of Cit3−. Biocompatibility study indicates that when co-cultured with C6 glioma cells in vitro for 3 days, ≤800 ppm Cit/Tb-nHAp is not cytotoxic for C6 glioma cells. We also present in vitro data showing efficient cytoplasmic localization of transferrin conjugated Cit/Tb-nHAp into C6 glioma cells by fluorescence cell imaging. We have successfully engineered Cit/Tb-nHAp, a promising biocompatible agent for future in vitro and in vivo fluorescence bioimaging. |
| Databáze: | OpenAIRE |
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