Structural and motional properties of vesicles as revealed by nuclear magnetic resonance
| Autor: | David B. Fenske |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Magnetic Resonance Spectroscopy
Vesicle Organic Chemistry Molecular Conformation technology industry and agriculture Phospholipid Cell Biology Phosphatidic acid Nuclear Overhauser effect Models Biological Biochemistry law.invention chemistry.chemical_compound Nuclear magnetic resonance chemistry law Phosphatidylcholine Liposomes Phosphatidylcholines lipids (amino acids peptides and proteins) Lipid bilayer Electron paramagnetic resonance Molecular Biology Two-dimensional nuclear magnetic resonance spectroscopy Mathematics |
| Zdroj: | Chemistry and Physics of Lipids. 64:143-162 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/0009-3084(93)90063-9 |
| Popis: | Correspondence to: David B. Fenske, Department of Biochemistry, Faculty of Medicine, University of British Columbia, 2146 Health Sciences Mall, Vancouver, B.C., Canada V6T 1Z3. Abbreviations: CHOL, cholesterol; CL, cardiolipin; D, lateral diffusion coefficient; DMPC, L-t~-dimyristoyl phosphatidylcholine; DOPA, L-ct-dioleoyl phosphatidic acid; DOPC, L-c~dioleoyl phosphatidylcholine; DOPE, L-ct-dioleoyl phosphatidylethanolamine; DOPS, L-~-dioleoyl phosphatidylserine; I)PPC, L-t~-dipalmitoyl phosphatidylcholine; DSC, differential scanning calorimetry; DSPC, t-a-distearoyl phosphatidylcholine; DSPG, L-c~-distearoyl phosphatidylglycerol; EPC, egg phosphatidylcholine; ESR, electron spin resonance; FRAP; fluorescence recovery after photobleaching; FT-IR, Fourier transform infrared spectroscopy; Gd-DTPA, gadoliniumdiethylenetriamine-pentaacetic acid; HDL, high density lipoprotein; LDL, low density lipoprotein; LPC, lysophosphatidylcholine; LUV, large unilamellar vesicle; MLV, multilamellar vesicle; MRI, magnetic resonance imaging; NOESY, nuclear Overhauser enhancement and exchange spectroscopy; NMR, nuclear magnetic resonance; NMRD, nuclear magnetic relaxation dispersion; NOE, nuclear Overhauser enhancement; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; P1, phosphatidylinositol; PIP, PI-4-phosphate; PIP 2, PI-4,5-diphosphate; PS, phosphatidylserine; POPC, lpalmitoyl-2-oleoyl phosphatidylcholine; POPC-d31 , l-[2H31]Palmitoyl-2-oleoyl phosphatidylcholine; QELS, quasi-elastic light scattering; ROESY, rotating frame nuclear Overhauser effect spectroscopy; SCD , carbon-deuterium bond order parameter; SPM, sphingomyelin; SUV, small unilamellar vesicle; TI, spin-lattice or longitudinal relaxation time; T2, spin-spin or transverse relaxation time; Tin, gel-to-liquid crystalline phase transition temperature; VLDL, very low density lipoprotein. One of the most powerful techniques that has been applied to the study of biological and model membranes is nuclear magnetic resonance (NMR). Questions regarding both static and dynamic aspects of membrane structure can be probed via an almost limitless number of pulse sequences, thereby providing a wealth of information not easily obtained from other techniques. One reason for the usefulness of NMR in the elucidation of membrane structure stems from the anisotropic nature of molecular motions within liquidcrystalline lipid bilayers. This is true for intramolecular reorientations of lipid functional groups (acyl chains, headgroups) as well as motions of the whole molecule (rotational and lateral diffusion, transbilayer transport). NMR allows characterization of these motions because many magnetic interactions are themselves anisotropic (e.g., the chemical shift anisotropy, dipolar and quadrupolar interactions). Information on molecular ordering and orientation, rate and type of motion(s) and polymorphism have been obtained from such techniques as solid-state 2H[1-6] 31p_ [2,7-9] and 13C-NMR [2]. Unlike other methods such as fluorescence and ESR which rely on bulky reporter groups, NMR is entirely non-perturbing. A wide range of nuclei are available for study |
| Databáze: | OpenAIRE |
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