Culture-Based Quantification with Molecular Characterization of Non-O157 and O157 Enterohemorrhagic Escherichia coli Isolates from Rectoanal Mucosal Swabs of Feedlot Cattle
| Autor: | Rodney A. Moxley, Liesel G Schneider, Galen E. Erickson, David R. Smith, Zachary R. Stromberg, Gentry L. Lewis, Isha R. Patel |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine food.ingredient 030106 microbiology Virulence Escherichia coli O157 Serogroup medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Shiga Toxin Agar plate Feces 03 medical and health sciences food hemic and lymphatic diseases Multiplex polymerase chain reaction medicine Animals Agar Escherichia coli Intimin biology Escherichia coli Proteins Shiga toxin biochemical phenomena metabolism and nutrition bacterial infections and mycoses 030104 developmental biology Enterohemorrhagic Escherichia coli biology.protein bacteria Cattle Animal Science and Zoology Multiplex Polymerase Chain Reaction Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 15:26-32 |
| ISSN: | 1556-7125 1535-3141 |
| Popis: | Enterohemorrhagic Escherichia coli (EHEC) strains are foodborne pathogens carried in the intestinal tracts of ruminants and shed in the feces. High concentrations (≥104 colony-forming units [CFU]/g) of EHEC in cattle feces are associated with contamination of hides, and subsequently, carcasses and beef. Several studies using agar media have quantified O157 but few have quantified non-O157 EHEC in samples from cattle. Thus, the objective of this study was to determine the concentration of O157 and non-O157 EHEC in cattle, and to characterize the associated EHEC isolates for their virulence potential. Two hundred feedlot steers were sampled by rectoanal mucosal swab (RAMS) every 35 days over four sampling periods, and a spiral plating method using modified Posse differential agar was used to quantify EHEC organisms in these samples. Bacterial colonies from agar plates were tested by multiplex PCR for Shiga toxin and intimin genes (stx and eae, respectively), and confirmed EHEC isolates (i.e., posit... |
| Databáze: | OpenAIRE |
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