Identification of 3'UTR sequence elements and a teloplasm localization motif sufficient for the localization of Hro-twist mRNA to the zygotic animal and vegetal poles
| Autor: | Hoan V. Tran, Stephanie A. Mandal, Christine Nelson, Agustin I. Seoane, Roberto A. Lleras, Mehrin Farooq, Julio G. Soto, Jonathan Choi |
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| Rok vydání: | 2012 |
| Předmět: |
Mesoderm
RNA Folding animal structures Embryo Nonmammalian Zygote Cytoplasmic polyadenylation element Embryonic Development Cell fate determination Protein Structure Secondary RNA Transport Article Leeches medicine Animals Point Mutation RNA Messenger Cloning Molecular Nucleotide Motifs Structural motif 3' Untranslated Regions Body Patterning Repetitive Sequences Nucleic Acid Messenger RNA biology Three prime untranslated region Twist-Related Protein 1 Computational Biology Gene Expression Regulation Developmental Cell Biology Stem-loop biology.organism_classification Molecular biology medicine.anatomical_structure Helobdella robusta Mutagenesis Site-Directed Developmental Biology |
| Zdroj: | Development, growthdifferentiation. 54(4) |
| ISSN: | 1440-169X |
| Popis: | The early localization of mRNA transcripts is critical in sorting cell fate determinants in the developing embryo. In the glossiphoniid leech, Helobdella robusta, maternal mRNAs, such as Hro-twist, localize to the zygotic teloplasm. Ten seven nucleotide repeat elements (AAUAAUA) called ARE2 and a predicted secondary structural motif, called teloplasm localization motif (TLM), are present in the 3′UTR of Hro-twist mRNA. We used site-directed mutagenesis, deletions, and microinjection of labeled, exogenous transcripts to determine if ARE2 elements, and the TLM, play a role in Hro-twist mRNA localization. Deleting the poly-A tail and the cytoplasmic polyadenylation element (CPE) had no effect on Hro-twist mRNA localization. Site-directed mutagenesis of nucleotides that altered ARE2 element sequences or the TLM suggest that the ARE2 elements and the TLM are important for Hro-twist mRNA localization to the teloplasm of pre-cleavage zygotes. Hro-Twist protein expression data suggest that the localization of Hro-twist transcripts in zygotes and stage two embryos is not involved in ensuring mesoderm specification, as Hro-Twist protein is expressed uniformly in most cells before gastrulation. Our data may support a shared molecular mechanism for leech transcripts that localize to the teloplasm. |
| Databáze: | OpenAIRE |
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