Spontaneously immortalized mouse mesothelial cells display characteristics of malignant transformation
| Autor: | Amanda Sherwood, Nigel Swanson, Steve E. Mutsaers, Violet K. Peeva, Richard A. Lake, Cleo Robinson, Christopher J.S. deSilva |
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| Rok vydání: | 2008 |
| Předmět: |
Mesothelioma
Population Down-Regulation Biology Malignant transformation Mice Gene expression Animals Humans Genes Tumor Suppressor education Cell Shape Cellular Senescence Cell Line Transformed education.field_of_study Gene Expression Profiling Contact inhibition Epithelial Cells Original Articles DNA Cell Biology General Medicine In vitro Cell biology Mice Inbred C57BL Transformation (genetics) Cell Transformation Neoplastic Cell culture Immunology Intercellular Signaling Peptides and Proteins Gene Deletion Mesothelial Cell |
| Zdroj: | Cell Proliferation. 41:894-908 |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/j.1365-2184.2008.00560.x |
| Popis: | Objectives: Mesotheliomas occur in occult serous cavities after chronic exposure of mesothelial cells to asbestos fibres. Molecular events that contribute to the development of this cancer are therefore not readily accessible for study. We have used in vitro culture systems to study and compare induced and spontaneous transformation events in primary mouse mesothelial cells. Materials and methods: Mouse mesothelial cells were cultivated until small populations of proliferating cells emerged from senescing cultures. Spontaneously transformed cultures of cells were characterized and compared to malignantly transformed cells. Results: Human mesothelial cells had a finite lifespan of 10–15 population doublings when cultured in vitro; mouse mesothelial cells typically exhibit this same pattern. Here, we show that mouse mesothelial cells can be cultured for extended periods and that these cells can transform spontaneously. Lines of spontaneously transformed cells generated in this study are immortal and growth factor‐independent. They display the salient characteristic features of transformation, including increased proliferation rate, lack of contact inhibition, aneuploidy and ability to grow in anchorage‐independent conditions. A subset of these cell lines developed into tumours in syngeneic mice. Comparative gene expression analysis demonstrated that spontaneously transformed cell lines were more closely related to neoplastic cells than to primary cells. Conclusion: These findings have implications for interpretation of in vitro transformation studies, demonstrating broad similarity between spontaneous and induced genetic changes. |
| Databáze: | OpenAIRE |
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