Seronegative HIV-2 carriers in India
| Autor: | Hideaki Tsuchie, Zhang J, K Shiraki, Janak K. Maniar, A Tanabe-Tochikura, Taniguchi K, Seiji Kageyama, M. Iwasaki, Takashi Kurimura, D G Saple |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
T-Lymphocytes
India Dermatology Polymerase Chain Reaction Virus law.invention Cell Line Immune system Antigen law HIV Seronegativity HIV Seropositivity Humans Pharmacology (medical) Fluorescent Antibody Technique Indirect Polymerase chain reaction biology Public Health Environmental and Occupational Health virus diseases Viral Load biology.organism_classification Virology Infectious Diseases Cell culture Immunology Lentivirus Carrier State DNA Viral HIV-2 biology.protein Antibody Viral load |
| Zdroj: | International journal of STDAIDS. 11(1) |
| ISSN: | 0956-4624 |
| Popis: | The discordant cases of seronegative, but culture and proviral HIV-2 DNA positive were found in Mumbai, India. This was corroborated by the successful isolation of HIV-2-RNA in culture medium, HIV-2 cDNA sequence determination and the detection of the antigen. The sequence of the isolated HIV-2 genomic RNA does not seem to be altered to the extent that the change will alter antibody binding. Furthermore, antibody from the same individual (even at 8 months from initial sampling) from whom HIV-2 was isolated did not react with the antigen of this strain. Those evidences imply that extremely low or non-production of the antibody may be due to suboptimal immune stimulation due to extremely slow HIV-2 replication. This low virus-load may be responsible for the negative antibody results in the HIV-2 carriers.This paper describes the characteristics of HIV-2 seropositive and seronegative cases in Mumbai, India, and characterizes the differences between HIV-1 and HIV-2. More than 200 outpatients considered to be at high risk of HIV infection were screened for HIV-1 and HIV-2 antibody and proviral DNA. The study found 11 cases that were discordant for antibody test and HIV proviral DNA (i.e., negative for anti-HIV but positive for HIV-2 proviral DNA). The presence of this provirus was further corroborated by the detection of HIV-2 RNA in the culture medium upon HIV isolation, HIV-2 cDNA sequencing, and antigen detection. The sequence of the isolated HIV-2 genomic RNA did not seem to be altered to the extent that the change would affect antibody binding. Moreover, antibody from the same person in whom HIV-2 was detected did not react with the antigen of this strain even 8 months after the initial sampling. These findings indicate that extremely low production or non-production of the antibody may be brought about by suboptimal immune stimulation due to very low HIV-2 replication speed. This low virus load may account for the negative antibody results in the HIV-2 carriers in India. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|