Urokinase Receptor Counteracts Vascular Smooth Muscle Cell Functional Changes Induced by Surface Topography
| Autor: | Hermann Haller, Kestutis Kurselis, Boris N. Chichkov, Roman Kiyan, Inna Dumler, Yulia Kiyan |
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| Rok vydání: | 2013 |
| Předmět: |
Vascular smooth muscle
Cytoskeleton organization Medicine (miscellaneous) Muscle Smooth Vascular connexin 43 Tissue engineering microstructured biomaterial cell elongation Pharmacology Toxicology and Pharmaceutics (miscellaneous) Cells Cultured cellular distribution Chemistry article cell communication cytoskeleton Anatomy protein function Cell biology Vascular smooth muscle cell cardiovascular system cell transdifferentiation Research Paper Cell signaling phenotype polymer Myocytes Smooth Muscle spindle cell protein localization Vascular injury cell shape Focal adhesion Downregulation and upregulation urokinase receptor Humans controlled study ddc:610 human Cell adhesion protein expression Focal Adhesions human cell focal adhesion kinase cell adhesion Urokinase-Type Plasminogen Activator Urokinase receptor Microstructured biomaterial cell proliferation vascular smooth muscle smooth muscle actin smooth muscle fiber cell function Dewey Decimal Classification::600 | Technik::610 | Medizin Gesundheit upregulation cell structure |
| Zdroj: | Theranostics 3 (2013), Nr. 7 Theranostics |
| ISSN: | 1838-7640 |
| DOI: | 10.7150/thno.4119 |
| Popis: | Molecular beacons (MBs) of DCurrent treatments for human coronary artery disease necessitate the development of the next generations of vascular bioimplants. Recent reports provide evidence that controlling cell orientation and morphology through topographical patterning might be beneficial for bioimplants and tissue engineering scaffolds. However, a concise understanding of cellular events underlying cell-biomaterial interaction remains missing. In this study, applying methods of laser material processing, we aimed to obtain useful markers to guide in the choice of better vascular biomaterials. Our data show that topographically treated human primary vascular smooth muscle cells (VSMC) have a distinct differentiation profile. In particular, cultivation of VSMC on the microgrooved biocompatible polymer E-shell induces VSMC modulation from synthetic to contractile phenotype and directs formation and maintaining of cell-cell communication and adhesion structures. We show that the urokinase receptor (uPAR) interferes with VSMC behavior on microstructured surfaces and serves as a critical regulator of VSMC functional fate. Our findings suggest that microtopography of the E-shell polymer could be important in determining VSMC phenotype and cytoskeleton organization. They further suggest uPAR as a useful target in the development of predictive models for clinical VSMC phenotyping on functional advanced biomaterials.NA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluoro-phores that have been employed to sense DNA/RNA. With different probes, we epitomize the fundamental understanding of driving forces and these recognition processes. Moreover, we will introduce a few novel and attractive emerging applications and discuss their advantages and dis-advantages. We also highlight several perspective probes in the application of cancer therapeutics. |
| Databáze: | OpenAIRE |
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