Fasting induced kisspeptin signaling suppression is regulated by glutamate mediated cues in adult male rhesus macaque (Macaca mulatta)
| Autor: | Nadia Shabbir, Hira Zubair, Shazia Shamas, Saeed-ul-Hassan Khan, Muhammad Yousaf Khan, Saira Shafqat, Muhammad Shahab, Fazal Wahab |
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| Rok vydání: | 2015 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Glutamate decarboxylase Hypothalamus Glutamic Acid Biology Receptors N-Methyl-D-Aspartate Receptors G-Protein-Coupled Cellular and Molecular Neuroscience Glutamatergic Endocrinology Kisspeptin Internal medicine medicine Animals Testosterone gamma-Aminobutyric Acid Neurons Kisspeptins Endocrine and Autonomic Systems Glutamate Decarboxylase Glutamate receptor General Medicine Fasting biology.organism_classification Macaca mulatta Rhesus macaque nervous system Neurology GABAergic NMDA receptor Cues hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Neuropeptides. 52 |
| ISSN: | 1532-2785 |
| Popis: | Kisspeptin signaling is suppressed by short term fasting. It has been reported that hypothalamic Kiss1 and Kiss1r mRNA expression decreased after 48h of fasting in male rhesus monkey. But the mechanism involved in the reduction of kisspeptin signaling after 48h of fasting is unknown. Recent studies have suggested the role of afferent excitatory and inhibitory pathways in the regulation of kisspeptin neurons. Therefore, this study was designed to observe the changes in the glutamate and GABA signaling during fed and 48h fasting states by performing immunofluorescence to examine the interaction of kisspeptin neurons with NR1 subunit of NMDA receptors and by performing SYBR green qRT-PCR to measure and quantify the levels of Kiss1, Kiss1r, NR1 and GAD67 mRNA in the POA and MBH of adult male rhesus macaque (Macaca mulatta) during 48h of fasting (n=2) and fed ad libitum (n=2). Plasma testosterone (p |
| Databáze: | OpenAIRE |
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