RNA-Seq provides new insights on the relative mRNA abundance of antioxidant components during mouse liver development
| Autor: | Julia Yue Cui, Curtis D. Klaassen, Kai Connie Wu, Hong Lu, Xiao-bo Zhong, Jie Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Antioxidant medicine.medical_treatment Biology Biochemistry Antioxidants Article Transcriptome Superoxide dismutase Mice 03 medical and health sciences 0302 clinical medicine Physiology (medical) medicine Animals Metallothionein RNA Messenger RNA-Seq Messenger RNA Gene Expression Profiling Alternative splicing Gene Expression Regulation Developmental KEAP1 Mice Inbred C57BL 030104 developmental biology Liver Catalase biology.protein Female 030217 neurology & neurosurgery |
| Zdroj: | Free Radical Biology and Medicine. 134:335-342 |
| ISSN: | 0891-5849 |
| Popis: | Mammals have developed a variety of antioxidant systems to protect them from the oxygen environment and toxic stimuli. Little is known about the mRNA abundance of antioxidant components during postnatal development of the liver. Therefore, the purpose of this study was to compare the mRNA abundance of antioxidant components during liver development. Livers from male C57BL/6J mice were collected at 12 ages from prenatal to adulthood. The transcriptome was determined by RNA-Seq with transcript abundance estimated by Cufflinks. RNA-Seq provided a complete, more accurate, and unbiased quantification of the transcriptome. Among 33 known antioxidant components examined, three ontogeny patterns of liver antioxidant components were observed: (1) Prenatal-enriched, in which the mRNAs decreased from fetal livers to adulthood, such as metallothionein and heme oxygenase-1; (2) adolescent-rich and relatively stable expression, such as peroxiredoxins; and (3) adult-rich, in which the mRNA increased with age, such as catalase and superoxide dismutase. alternative splicing of several antioxidant genes, such as Keap1, Glrx2, Gpx3, and Txnrd1, were also detected by RNA-Seq. In summary, RNA-Seq revealed the relative abundance of hepatic antioxidant enzymes, which are important in protecting against the deleterious effects of oxidative stress. |
| Databáze: | OpenAIRE |
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