Comb-Like Fluorophilic-Lipophilic-Hydrophilic Polymers for Nanocapsules as Ultrasound Contrast Agents
| Autor: | Nicolas Huang, Nicolas Tsapis, Laurence Moine, Olivier Couture, Guilherme Fadel Picheth, Sophie Houvenagel, Annie Brûlet, Vincent Faugeras, Camille Dejean, Alexis Chennevière |
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| Přispěvatelé: | Institut Galien Paris-Sud (IGPS), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Léon Brillouin (LLB - UMR 12), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Polymers and Plastics Fluorine Compounds Contrast Media Bioengineering macromolecular substances 02 engineering and technology 010402 general chemistry Methacrylate 01 natural sciences Nanocapsules Cell Line Polyethylene Glycols Biomaterials chemistry.chemical_compound Mice Materials Chemistry Copolymer Human Umbilical Vein Endothelial Cells Animals Humans Ultrasonography chemistry.chemical_classification Atom-transfer radical-polymerization Macrophages technology industry and agriculture Polymer Permeation 021001 nanoscience & nanotechnology 0104 chemical sciences [CHIM.POLY]Chemical Sciences/Polymers Polymerization chemistry Chemical engineering Acrylates 0210 nano-technology Ethylene glycol |
| Zdroj: | Biomacromolecules Biomacromolecules, American Chemical Society, 2018, 19 (8), pp.3244-3256. ⟨10.1021/acs.biomac.8b00506⟩ Biomacromolecules, 2018, 19 (8), pp.3244-3256. ⟨10.1021/acs.biomac.8b00506⟩ |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.8b00506⟩ |
| Popis: | International audience; Imaging the enhanced permeation and retention effect by ultrasound is hindered by the large size of commercial ultrasound contrast agents (UCAs). To obtain nanosized UCAs, triblock copolymers of poly(ethylene glycol)-polylactide-poly(1H,1H,2H,2H-heptadecafluorodecyl methacrylate) (PEG-PLA-PFMA) with distinct numbers of perfluorinated pendant chains (5, 10, or 20) are synthesized by a combination of ring-opening polymerization and atom transfer radical polymerization. Nanocapsules (NCs) containing perfluorooctyl bromide (PFOB) intended as UCAs are obtained with a 2-fold increase in PFOB encapsulation efficiency in fluorinated NCs as compared with plain PEG-PLA NCs thanks to fluorous interactions. NC morphology is strongly influenced by the number of perfluorinated chains and the amount of polymer used for formulation, leading to peculiar capsules with several PFOB cores at high PEG-PLA-PFMA 20 amount and single-cored NCs with a thinner shell at low fluorinated polymer amount, as confirmed by small-angle neutron scattering. Finally, fluorinated NCs yield higher in vitro ultrasound signal compared with PEG-PLA NCs, and no in vitro cytotoxicity is induced by fluorinated polymers and their degradation products. Our results highlight the benefit of adding comb-like fluorinated blocks in PEG-PLA polymers to modify the nanostructure and enhance the echogenicity of nanocapsules intended as UCAs. |
| Databáze: | OpenAIRE |
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