Increased susceptibility of microcytic red blood cells to in vitro oxidative stress
| Autor: | M. T. Dibarrart, Pujades Ma, M. A. Calvo, Mariano Linares, A. Miguel-Garcia, A. Miguel-Sosa, S. Cambiazzo, J. L. Vives Corrons |
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| Rok vydání: | 1995 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities medicine.medical_specialty Erythrocytes Abnormal medicine.disease_cause Superoxide dismutase chemistry.chemical_compound hemic and lymphatic diseases Internal medicine Malondialdehyde medicine Humans 5'-Nucleotidase chemistry.chemical_classification Anemia Hypochromic Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Microcytosis Hematology General Medicine Glutathione Iron deficiency medicine.disease Red blood cell Oxidative Stress Endocrinology medicine.anatomical_structure chemistry Iron-deficiency anemia Immunology biology.protein Thalassemia Reactive Oxygen Species Oxidative stress |
| Zdroj: | European journal of haematology. 55(5) |
| ISSN: | 0902-4441 |
| Popis: | Oxidative damage to erythrocytes in thalassaemia has been related to generation of free radicals by an excess of denaturated alpha- or beta-globin chains, intracellular iron overload and low concentration of normal haemoglobin (HGB). Two good indicators of such oxidative damage are the high red blood cell (RBC) malonyldialdehyde (MDA) production detected following exogenous oxidant stress and the decrease of pyrimidine 5'-nucleotidase (P5N), the most sensitive enzyme to SH-group damage in vivo. Conflicting data, however, have so far accumulated in the literature concerning differences in oxidative damage between the different forms of thalassaemia and iron deficiency anaemia (IDA). In the present study, oxidative susceptibility, as defined by the production of MDA in vitro and antioxidant capacity, as measured by the activity of RBC glutathione peroxidase (GPx), superoxide dismutase (SOD) and by reduced glutathione (GSH), have been studied in microcytic RBCs from patients with beta-thalassaemia trait, Spanish (delta beta) zero-thalassaemia heterozygotes (delta beta-thalassaemia trait) and iron deficiency anaemia (IDA). The results are consistent with the existence of significant differences in the severity and pattern of oxidative stress susceptibility between beta-thalassaemia trait (increased MDA production and higher SOD and GPx activities) and the other two forms of microcytosis (delta beta thalassaemia trait and IDA). Furthermore, the finding of normal P5' N activity in delta beta thalassaemia trait, gives further support to the less intense peroxidative environment of RBCs in this form of thalassaemia when compared to beta-thalassaemia trait, characterized by acquired RBC P5' N deficiency due to oxidative damage. |
| Databáze: | OpenAIRE |
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