Matrix metalloproteinase-2 is involved in A549 cell migration on laminin-10/11
| Autor: | Ryoko Nishiuchi, Kiyotoshi Sekiguchi, Jianguo Gu |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Integrins
Matrix metalloproteinase inhibitor Integrin Biophysics Matrix metalloproteinase Matrix Metalloproteinase Inhibitors Biochemistry Antibodies Laminin Cell Movement Tumor Cells Cultured Humans Protease Inhibitors Molecular Biology A549 cell biology Chemistry Integrin alpha3beta1 Cell migration Cell Biology Cell biology Fibronectin Microscopy Fluorescence biology.protein Matrix Metalloproteinase 2 |
| Zdroj: | Biochemical and biophysical research communications. 296(1) |
| ISSN: | 0006-291X |
| Popis: | We have reported that laminin-10/11 strongly promotes migration of A549 human lung carcinoma cells by activating the alpha3beta1 integrin-dependent signaling pathway. To elucidate the mechanism involved, we investigated whether matrix metalloproteinases (MMPs) are involved in cell migration on laminin-10/11. Here, we demonstrate that laminin-10/11, but not fibronectin which does not greatly promote A549 cell movement, stimulated MMP-2 secretion approximately 3-fold. The cell migration-promoting activity of laminin-10/11 was down-regulated by an MMP inhibitor. In addition, cell motility was significantly increased when cells adhered to a mixture of fibronectin and laminin-10/11 with a concomitant decrease of focal contacts, compared with those adhering to fibronectin alone. The enhanced cell migration was partially suppressed by the MMP inhibitor. Furthermore, an anti-alpha3 integrin, but not an anti-alpha5 integrin, antibody induced the activated form of MMP-2. These data suggest that MMP-2 may play an important role in A549 cell migration on laminin-10/11 through an alpha3beta1 integrin-dependent pathway. |
| Databáze: | OpenAIRE |
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