The embryonic ontogeny of the gonadal somatic cells in mice and monkeys
| Autor: | Yasuhiro Murakawa, Takuya Yamamoto, Yukihiro Yabuta, Ikuhiro Okamoto, Mitinori Saitou, Akiko Oguchi, Chizuru Iwatani, Hiroshi Ohta, Kotaro Sasaki, Keren Cheng, Yasunari Seita, Hideaki Tsuchiya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine endocrine system hox genes Lineage (genetic) monkey gonadogenesis QH301-705.5 Mesenchyme Ovary Biology anterior-posterior regionalization posterior intermediate mesoderm General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals intermediate mesoderm Biology (General) Gonads Hox gene adrenal gland Gonadal ridge bipotential gonad Primitive streak Cell Differentiation primate gonadogenesis Embryonic stem cell Cell biology genital ridge Adult Stem Cells Macaca fascicularis 030104 developmental biology medicine.anatomical_structure single-cell transcriptomics Intermediate mesoderm 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 35, Iss 5, Pp 109075-(2021) |
| ISSN: | 2211-1247 |
| Popis: | Summary: In the early fetal stage, the gonads are bipotent and only later become the ovary or testis, depending on the genetic sex. Despite many studies examining how sex determination occurs from biopotential gonads, the spatial and temporal organization of bipotential gonads and their progenitors is poorly understood. Here, using lineage tracing in mice, we find that the gonads originate from a T+ primitive streak through WT1+ posterior intermediate mesoderm and appear to share origins anteriorly with the adrenal glands and posteriorly with the metanephric mesenchyme. Comparative single-cell transcriptomic analyses in mouse and cynomolgus monkey embryos reveal the convergence of the lineage trajectory and genetic programs accompanying the specification of biopotential gonadal progenitor cells. This process involves sustained expression of epithelial genes and upregulation of mesenchymal genes, thereby conferring an epithelial-mesenchymal hybrid state. Our study provides key resources for understanding early gonadogenesis in mice and primates. |
| Databáze: | OpenAIRE |
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