A rise in nuclear calcium translocates annexins IV and V to the nuclear envelope
| Autor: | Eduardo Rojas, Harvey B. Pollard, Gemma A. J. Kuijpers, Patrick Raynal |
|---|---|
| Přispěvatelé: | Centre d’Etudes et de Recherches en Psychopathologie et Psychologie de la Santé (CERPPS), Université Toulouse - Jean Jaurès (UT2J) |
| Rok vydání: | 1996 |
| Předmět: |
Nuclear Envelope
[SDV]Life Sciences [q-bio] Biophysics Ionophore Chromosomal translocation Annexin Biology Bradykinin Immunofluorescence Biochemistry Nucleus Phospholipases A 03 medical and health sciences Cytosol 0302 clinical medicine Structural Biology Genetics medicine Extracellular Animals Humans Annexin A5 Annexin A4 Molecular Biology Calcimycin ComputingMilieux_MISCELLANEOUS 030304 developmental biology Cell Nucleus Calcium metabolism 0303 health sciences Ionophores medicine.diagnostic_test 85-kDa cytosolic phospholipase A2 Cell Biology Fibroblasts Rats Cell biology Ca2+ medicine.anatomical_structure Microscopy Fluorescence 030220 oncology & carcinogenesis Calcium Annexin A2 |
| Zdroj: | FEBS Letters FEBS Letters, Wiley, 1996, 392 (3), pp.263-268. ⟨10.1016/0014-5793(96)00827-7⟩ |
| ISSN: | 0014-5793 1873-3468 |
| DOI: | 10.1016/0014-5793(96)00827-7 |
| Popis: | Following incubation of human fibroblasts with Ca2+ ionophore A23187, we found strong immunofluorescence labelling of the nuclear envelope by annexin IV antibody. Using confocal imaging of cells loaded with Fluo-3, we showed that A23187 generates an intense and sustained rise of Ca2+ in the nucleus. By contrast, stimulation without extracellular Ca2+ produces only a brief rise in nuclear Ca2+ that does not promote annexin IV translocation to the nuclear envelope, and compounds that induce only a transient increase of nuclear Ca2+ do not support translocation of annexin IV. In addition, annexin V was also translocated to the nuclear envelope by A23187, but distribution of annexins I, II, VI and VII is unaffected. In in vitro assays with isolated nuclei, annexin V was also found to bind to the nuclear envelope in a Ca2+-dependent manner. These results demonstrate that the translocation to the nuclear envelope of different types of Ca2+-regulated proteins is directly triggered by a major rise of Ca2+ in the nucleus. |
| Databáze: | OpenAIRE |
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