Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum
| Autor: | Nathaniel Nyakaat Ninyio, Ayesha Fatima, Siew Wei Lee, Adrian Tandhyka Gemiarto, Eric Wei Chiang Chan, Joko Logis, Crystale Siew Ying Lim |
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| Rok vydání: | 2015 |
| Předmět: |
food.ingredient
Indoles medicine.drug_class Antibiotics Microbiology Propolis chemistry.chemical_compound food Caffeic Acids Biosynthesis medicine Agar Molecular Biology Microbial Viability biology Virulence Chromobacterium Gene Expression Profiling Quorum Sensing General Medicine biology.organism_classification Biosynthetic Pathways Molecular Docking Simulation Quorum sensing chemistry Bee pollen Chromobacterium violaceum Bacteria |
| Zdroj: | Antonie van Leeuwenhoek. 108(2) |
| ISSN: | 1572-9699 |
| Popis: | The emergence of antibiotic-resistant bacterial pathogens, especially Gram-negative bacteria, has driven investigations into suppressing bacterial virulence via quorum sensing (QS) inhibition strategies instead of bactericidal and bacteriostatic approaches. Here, we investigated several bee products for potential compound(s) that exhibit significant QS inhibitory (QSI) properties at the phenotypic and molecular levels in Chromobacterium violaceum ATCC 12472 as a model organism. Manuka propolis produced the strongest violacein inhibition on C. violaceum lawn agar, while bee pollen had no detectable QSI activity and honey had bactericidal activity. Fractionated manuka propolis (pooled fraction 5 or PF5) exhibited the largest violacein inhibition zone (24.5 ± 2.5 mm) at 1 mg dry weight per disc. In C. violaceum liquid cultures, at least 450 µg/ml of manuka propolis PF5 completely inhibited violacein production. Gene expression studies of the vioABCDE operon, involved in violacein biosynthesis, showed significant (≥two-fold) down-regulation of vioA, vioD and vioE in response to manuka propolis PF5. A potential QSI compound identified in manuka propolis PF5 is a hydroxycinnamic acid-derivative, isoprenyl caffeate, with a [M−H] of 247. Complete violacein inhibition in C. violaceum liquid cultures was achieved with at least 50 µg/ml of commercial isoprenyl caffeate. In silico docking experiments suggest that isoprenyl caffeate may act as an inhibitor of the violacein biosynthetic pathway by acting as a competitor for the FAD-binding pockets of VioD and VioA. Further studies on these compounds are warranted toward the development of anti-pathogenic drugs as adjuvants to conventional antibiotic treatments, especially in antibiotic-resistant bacterial infections. |
| Databáze: | OpenAIRE |
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