Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response

Autor: José Manuel Franco-Zorrilla, Li Shi, Lorena Ramírez Gonzales, Sara Bergonzi, Roberto Solano-Tavira, Richard G. F. Visser, Christian W. B. Bachem, José A. Abelenda, Marian Oortwijn
Přispěvatelé: Netherlands Organization for Scientific Research, Agencia Estatal de Investigación (España), Comunidad de Madrid, Ramírez Gonzales, Lorena [0000-0001-7052-8781], Bergonzi, Sara Bergonzi [0000-0002-4137-8064], Franco-Zorrilla, José M [0000-0001-6769-7349], Solano-Tavira, Roberto [0000-0001-5459-2417], Visser, Richard G F [0000-0002-0213-4016], Abelenda, José A [0000-0001-7952-4891], Bachem, Christian W B [0000-0002-3077-6833], Ramírez Gonzales, Lorena, Bergonzi, Sara Bergonzi, Franco-Zorrilla, José M, Solano-Tavira, Roberto, Visser, Richard G F, Abelenda, José A, Bachem, Christian W B
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Plant Journal, 105(4), 855-869
Plant Journal 105 (2021) 4
The Plant Journal
ISSN: 0960-7412
DOI: 10.1111/tpj.15093
Popis: Departamento de Biotecnología (INIA)
Plants regulate their reproductive cycles under the influence of environmental cues, such as day length, temperature and water availability. In Solanum tuberosum (potato), vegetative reproduction via tuberization is known to be regulated by photoperiod, in a very similar way to flowering. The central clock output transcription factor CYCLING DOF FACTOR 1 (StCDF1) was shown to regulate tuberization. We now show that StCDF1, together with a long non-coding RNA (lncRNA) counterpart, named StFLORE, also regulates water loss through affecting stomatal growth and diurnal opening. Both natural and CRISPR-Cas9 mutations in the StFLORE transcript produce plants with increased sensitivity to water-limiting conditions. Conversely, elevated expression of StFLORE, both by the overexpression of StFLORE or by the downregulation of StCDF1, results in an increased tolerance to drought through reducing water loss. Although StFLORE appears to act as a natural antisense transcript, it is in turn regulated by the StCDF1 transcription factor. We further show that StCDF1 is a non-redundant regulator of tuberization that affects the expression of two other members of the potato StCDF gene family, as well as StCO genes, through binding to a canonical sequence motif. Taken together, we demonstrate that the StCDF1-StFLORE locus is important for vegetative reproduction and water homeostasis, both of which are important traits for potato plant breeding.
This work was supported by the ERA CAPS HotSol project funded by the Netherlands Organization for Scientific Research (NWO project 849.13.001), the FlowerPot project, supported by the Division for Earth and Life Sciences (ALW) with financial aid from the NWO. JAA was a recipient of an European Molecular Biology Organization (EMBO) fellowship (ASTF 485-2013) to perform the initial part of the research. JAA also wants to recognize the ‘Severo Ochoa Program for Centres of excellence in R&D’ from the State Research Agency (AEI; grant SEV-2016-0672), together with the Comunidad de Madrid Atracción de Talento Investigador programme (grant 2018T1/BIO11380) for their current financial support.
Databáze: OpenAIRE
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