Antibiofilm Effect of Trans-Cinnamaldehyde on Uropathogenic Escherichia coli
| Autor: | Sangeetha Ananda Baskaran, Mary Anne Roshni Amalaradjou, Kumar Venkitanarayanan, Amoolya Narayanan |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.drug_class
Urology medicine.medical_treatment Antibiotics In Vitro Techniques medicine.disease_cause Bacterial Adhesion Tryptic soy broth Urinary catheterization Microbiology chemistry.chemical_compound Catheters Indwelling Humans Uropathogenic Escherichia coli Medicine Acrolein Escherichia coli Cells Cultured Escherichia coli Infections Microscopy Confocal biology business.industry Biofilm biology.organism_classification Enterobacteriaceae Catheter chemistry Biofilms Urinary Tract Infections Equipment Contamination Polystyrenes Factor Analysis Statistical Urinary Catheterization business Bacteria |
| Zdroj: | Journal of Urology. 184:358-363 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/j.juro.2010.03.006 |
| Popis: | Urinary tract infections are the most common hospital acquired infections in humans, caused primarily by uropathogenic Escherichia coli. Indwelling urinary catheters for bladder drainage in humans become encrusted with uropathogenic E. coli biofilms that are resistant to common antibiotics, resulting in chronic infections. We studied the efficacy of the cinnamon ingredient trans-cinnamaldehyde (Sigma) for preventing uropathogenic E. coli biofilm. We also determined the efficacy of trans-cinnamaldehyde as an ingredient in catheter lock solution to inactivate preformed uropathogenic E. coli biofilm.Polystyrene plates and urinary catheters inoculated with uropathogenic E. coli (5 to 6.0 log cfu) were treated with trans-cinnamaldehyde (0%, 0.1%, 0.25% or 0.5%) at 37C. Catheters with uropathogenic E. coli biofilm were also treated with lock solution containing trans-cinnamaldehyde (0%, 1%, 1.25% or 1.5%). Uropathogenic E. coli biofilm on control and trans-cinnamaldehyde treated plates and catheters was determined on incubation days 0, 1, 3 and 5. Trans-cinnamaldehyde potential cytotoxity, if any, was determined in HTB-4 bladder epithelial cells (ATCC).At all concentrations trans-cinnamaldehyde effectively prevented uropathogenic E. coli biofilm on plates and catheters. As a constituent in catheter lock solution, it inactivated uropathogenic E. coli biofilm on catheters. Trans-cinnamaldehyde produced no cytotoxic effects on human bladder epithelial cells at the tested concentrations.Results suggest that trans-cinnamaldehyde may be applied as a catheter surface coating or as an ingredient in catheter lock solution to prevent urinary tract infection in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|