Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
| Autor: | Saulo Duarte Ozelin, Flávia Aparecida Resende, Jacqueline Morais Alves, Ildercílio Mota de Souza Lima, Juliana Marques Senedese, Augusto Faria Andrade, Carla Carolina Munari, Eliana Aparecida Varanda, Denise Crispim Tavares, Jairo Kenupp Bastos, Débora L. Campos, Juliana de Carvalho da Costa |
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| Přispěvatelé: | Univ Franca, Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis antigenotoxicity Toxicology medicine.disease_cause Ames test 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Afzelin afzelin quercitrin Clonogenic assay 030304 developmental biology 0303 health sciences biology Traditional medicine fungi genotoxicity biology.organism_classification Quercitrin Copaifera langsdorffii Methyl methanesulfonate chemistry 030220 oncology & carcinogenesis Micronucleus test Genotoxicity |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-26T04:30:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-17 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Copaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin. Univ Franca, Lab Mutagenese, Franca, SP, Brazil Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo, Brazil Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, Brazil Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, Brazil FAPESP: 2011/1360-7 |
| Databáze: | OpenAIRE |
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