Identification of anthocyanin and other flavonoids from the green–blue petals of Puya alpestris (Bromeliaceae) and a clarification of their coloration mechanism
| Autor: | Moritoshi Iino, Takayuki Mizuno, Kohtaro Sugahara, Chie Tsutsumi, Naonobu Noda, Tsukasa Iwashina, Satoshi Koi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Bromeliaceae
0106 biological sciences Color Flowers Plant Science Horticulture 01 natural sciences Biochemistry Flavones Anthocyanins chemistry.chemical_compound Pigment Flavonols Botany Copigmentation Molecular Biology Flavonoids chemistry.chemical_classification biology 010405 organic chemistry General Medicine biology.organism_classification 0104 chemical sciences chemistry visual_art Anthocyanin visual_art.visual_art_medium Petal Delphinidin 010606 plant biology & botany Puya alpestris |
| Zdroj: | Phytochemistry. 181:112581 |
| ISSN: | 0031-9422 |
| Popis: | To understand the unique green–blue color of Puya alpestris (Bromeliaceae) flowers, we investigated their constituent anthocyanin and related compounds. An anthocyanin, two undescribed flavonols, and two flavones were isolated and identified as delphinidin 3,3′,5′-tri-O-β-glucopyranoside, myricetin 3-O-[α-rhamnopyranosyl-(1 → 6)-β-glucopyranoside]-3′,5′-di-O-β-glucopyranoside, myricetin 3,3′,5′-tri-O-β-glucopyranoside, luteolin 4′-O-glucoside, and apigenin 4′-O-glucoside. Furthermore, the presence of chlorophyll has also been revealed. P. alpestris petals show a gradient color appearance: Green–blue at the tip and blue at the base. This color difference between the tip and base was used to analyze the pigment components underlying the green–blue color expression. It was found that the petal tip contains the anthocyanin, flavonols, flavones, and chlorophyll in high quantities. Furthermore, the pH of petal juice was 6.2 and 5.6 at the tip and base, respectively. In vitro reconstruction revealed the blue color expression occurred via an intermolecular copigmentation between the anthocyanin and flavones, as well as yellow color expression, which was due to an increase in the absorption at 400–450 nm of the flavonols under the higher pH conditions. Furthermore, we found that the petal extract obtaining using 50% acetone containing chlorophyll showed the same absorption spectrum as that observed for the raw petal. These results indicate that the green–blue color of P. alpestris flowers is developed via an intermolecular co-pigmentation of the anthocyanin (delphinidin 3,3′,5′-tri-O-β-glucopyranoside) with flavones, such as luteolin 4′-O-glucoside, the yellow color expression of flavonols, such as myricetin 3,3′,5′-tri-O-glucoside under relatively high pH conditions in the cell sap, and the presence of chlorophyll. |
| Databáze: | OpenAIRE |
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