Correlation of probioticLactobacillus salivariusgrowth phase with its cell wall-associated proteome

Autor: Colum P. Dunne, Patricia B. Maguire, Mary Bennett, Gerald C. O'Sullivan, Achim Treumann, Richard Edwards, Fergus Shanahan, Desmond J. Fitzgerald, Peter Kelly, Bernd Thiede, J. Kevin Collins
Rok vydání: 2005
Předmět:
Zdroj: FEMS Microbiology Letters. 252:153-159
ISSN: 1574-6968
0378-1097
DOI: 10.1016/j.femsle.2005.08.051
Popis: Lactobacillus salivarius subsp. salivarius UCC118 is a probiotic bacterium that was originally isolated from human intestinal tissues and was subsequently shown in a pilot study to alleviate symptoms associated with mild-moderate Crohn's disease. Strain UCC118 can adhere to animal and human intestinal tissue, and to both healthy and inflamed ulcerative colitis mucosa, irrespective of location in the gut. In this study, an enzymatic technique has been combined with proteomic analysis to correlate bacterial growth phase with the presence of factors present in the cell wall of the bacterium. Using PAGE electrophoresis, it was determined that progression from lag to log to stationary growth phases in vitro correlated with increasing prominence of an 84 kD protein associated with in vitro adherence ability. Isolated proteins from the 84 kD band region were further separated by two-dimensional electrophoresis, resolving this band into 20 individual protein spots at differing isoelectric points. The protein moieties were excised, trypsin digested and subjected to tandem mass spectrometry. The observed proteins are analogous to those reported to be associated with the Listeria monocytogenes cell-wall proteome, and include DnaK, Ef-Ts and pyruvate kinase. These data suggest that at least some of the beneficial attributes of probiotic lactobacilli, and in particular this strain, may be due to nonpathogenic mimicry of pathogens and potentially be mediated through a form of attenuated virulence.
Databáze: OpenAIRE
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