Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China
| Autor: | Yang Zou, Jian-Fa Yang, Lian-Qin Shi, Sha-Sha Wang, Guang-Hui Zhao, Feng-Cai Zou, Dan Chen, Shi-Chen Xie, Zhao Li, Xing-Quan Zhu |
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| Rok vydání: | 2019 |
| Předmět: |
Giardiasis
Male 0301 basic medicine China Veterinary medicine Genotype 030231 tropical medicine Protozoan Proteins Yunnan Province Sheep Diseases Biology Host Specificity lcsh:Infectious and parasitic diseases Feces 03 medical and health sciences 0302 clinical medicine Glutamate Dehydrogenase Genetic variation Prevalence Animals Humans lcsh:RC109-216 Disease Reservoirs Goat Diseases Sheep Goats Research Black-boned sheep and goats Genetic Variation Baseline data Animal husbandry Multilocus genotypes (MLGs) Multilocus genotyping 030104 developmental biology Infectious Diseases Parasitology Genetic Loci Giardia duodenalis Female Giardia lamblia Nested polymerase chain reaction Multilocus Sequence Typing Triose-Phosphate Isomerase |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 12, Iss 1, Pp 1-9 (2019) |
| ISSN: | 1756-3305 |
| Popis: | Background Giardia duodenalis is an important intestinal protozoan infecting both humans and animals, causing significant public health concern and immeasurable economic losses to animal husbandry. Sheep and goats have been reported as common reservoirs of G. duodenalis, but only a limited amount of information is available for native breeds of these small ruminants in China. The present study investigated the prevalence and multilocus genotypes of G. duodenalis in black-boned sheep and goats, two important native breeds in Yunnan Province, southwestern China. Methods Fecal samples were collected from 336 black-boned goats and 325 black-boned sheep from five counties (Meishui, Shanshu, Shilin, Yongsheng and Nanping) of Yunnan Province and the genomic DNA was extracted from these feces. The prevalence of G. duodenalis was determined by the nested PCR targeting the β-giardin (bg) gene. The assemblages and multilocus genotypes (MLGs) were investigated based on analyses of three genetic loci, i.e. bg, glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi). Results Giardia duodenalis infection was detected in both black-boned sheep and goats, and the prevalence of G. duodenalis in black-boned sheep (21.8%, 71/325) was significantly higher (χ 2 = 36.63, df = 1, P |
| Databáze: | OpenAIRE |
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