Detection of basophil-activating IgG autoantibodies in chronic idiopathic urticaria by induction of CD63
| Autor: | Hugo Degreef, Ann De Swerdt, Anne-Marie Kochuyt, Pascal Cadot, Karen Neyens, Ahmad Kasran, Jan Ceuppens, Lieve Coorevits, Caroline Van Den Keybus |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Adolescent Urticaria Immunology chemical and pharmacologic phenomena Platelet Membrane Glycoproteins Basophil Immunoglobulin E medicine.disease_cause Immunoglobulin G Autoimmunity chemistry.chemical_compound Antigens CD parasitic diseases Humans Immunology and Allergy Medicine Autoantibodies Skin Tests biology Angioedema Receptors IgE Tetraspanin 30 business.industry Autoantibody hemic and immune systems Flow Cytometry Antibodies Anti-Idiotypic Basophils Basophil activation medicine.anatomical_structure chemistry Chronic Disease Immunologic Techniques biology.protein medicine.symptom business Histamine |
| Zdroj: | Journal of Allergy and Clinical Immunology. 116:662-667 |
| ISSN: | 0091-6749 |
| Popis: | Background Approximately 40% to 50% of patients with chronic idiopathic urticaria (CIU) have functional IgG autoantibodies against FcɛRIα or IgE, which induce histamine release from basophils and cutaneous mast cells. A positive autologous serum skin test response is believed to reflect the presence of these autoantibodies. Objective We sought to further define the functional properties of and develop a sensitive functional assay for detection of autoantibodies in patients with CIU. Methods Sera from patients with CIU (n=61) and sera from healthy control subjects (n=23) were incubated with donor basophils. Activation of basophils was determined on the basis of CD63 surface expression, as analyzed on a FACScan flow cytometer. Results A positive basophil activation test result was found in 51% of patients with CIU, and basophil-activating properties were present in the IgG fractions of sera. When both the in vitro test and the autologous serum skin test were considered, basophil/mast cell–activating autoantibodies were present in 62% of the patients. Patients with a positive basophil activation test result had a significantly higher prevalence of other autoantibodies, had more severe urticaria, and were more likely to have angioedema. Conclusion The results demonstrate the presence of basophil-activating autoantibodies in about 50% of patients with CIU. The data support the autoimmune cause of the disease and provide a simple test for detection of these autoantibodies. |
| Databáze: | OpenAIRE |
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