Hole-Burning Spectroscopy as a Probe of Nano-environments and Processes in Biomolecules: A Review
| Autor: | Peter Geissinger, Hannah E. Wagie |
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| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular Hydrostatic pressure Analytical chemistry Laser linewidth symbols.namesake Electromagnetic Fields Electric field Hydrostatic Pressure Animals Humans Spectroscopy Instrumentation chemistry.chemical_classification Quantitative Biology::Biomolecules Spectrum Analysis Biomolecule Proteins Energy landscape DNA Chromophore Nanostructures Stark effect chemistry Chemical physics symbols |
| Zdroj: | Applied Spectroscopy. 66:609-627 |
| ISSN: | 1943-3530 0003-7028 |
| Popis: | Hole-burning spectroscopy, a high-resolution spectroscopic technique, allows details of heterogeneous nano-environments in biological systems to be obtained from broad absorption bands. Recently, this technique has been applied to proteins, nucleic acids, cells, and substructures of water to probe the electrostatic conditions created by macromolecules and the surrounding solvent. Starting with the factors that obscure the homogeneous linewidth of a chromophore within an inhomogeneously broadened absorption or emission band, we describe properties and processes in biological systems that are reflected in the measured hole spectra. The technique also lends itself to the resolution of perturbation experiments, such as temperature cycling to elucidate energy landscape barriers, applied external electric fields (Stark effect) to measure net internal electric fields, and applied hydrostatic pressure to find the volume compressibility of proteins. |
| Databáze: | OpenAIRE |
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