Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases
| Autor: | Xiuhua Qu, Cheng Cao, Chenggong Li, Qincai Dong, Xuan Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Transcription Genetic Down-Regulation Biology 03 medical and health sciences Lab/In Vitro Research Transcription (biology) Cell Line Tumor hemic and lymphatic diseases Gene expression Cyclic AMP Humans Gene silencing Gene Silencing Phosphorylation KEGG Proto-Oncogene Proteins c-abl Transcription factor Cell Proliferation Genetics Regulation of gene expression Gene knockdown Neovascularization Pathologic Sequence Analysis RNA Gene Expression Profiling General Medicine Protein-Tyrosine Kinases Gene Expression Regulation Neoplastic Gene expression profiling 030104 developmental biology MCF-7 Cells Software Signal Transduction Transcription Factors |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.904888 |
| Popis: | BACKGROUND Studies have found that c-Abl oncogenic kinases may regulate gene transcription by RNA polymerase II phosphorylation or by direct regulation of specific transcription factors or coactivators. However, the global regulation of differential gene expression by c-Abl/Arg is largely unknown. In this study, differentially expressed genes (DEGs) regulated by c-Abl/Arg were identified, and related cellular functions and associated pathways were investigated. MATERIAL AND METHODS RNA obtained from wild-type and c-Abl/Arg gene-silenced MCF-7 cells was analyzed by RNA-Seq. DEGs were identified using edgeR software and partially validated by qRT-PCR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to explore the potential functions of these DEGs. RESULTS A total of 1,034 DEGs were significantly regulated by c-Abl/Arg (399 were up-regulated and 635 were down-regulated after c-Abl/Arg double knockdown). GO and KEGG analyses showed that the DEGs were primarily involved in cellular metabolic processes, neurodegenerative disease, the metabolic process and signaling pathway of cAMP, angiogenesis, and cell proliferation. CONCLUSIONS Our data collectively support the hypothesis that c-Abl/Arg regulate differential gene expression, providing new insights into the biological functions of c-Abl and Arg. |
| Databáze: | OpenAIRE |
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