| Popis: |
We have developed a new single-molecule fluorescence imaging analysis method, SIMA, that improves the temporal resolution of single-molecule localization and tracking studies to millisecond timescales without compromising the nanometer range spatial resolution [1,2,3,4]. In this method, the width of the fluorescence intensity profile of a static or a mobile molecule, imaged using submillisecond to milliseconds exposure time, is used for localization and dynamics analyses. We apply this method to three single-molecule investigations: (1) axial localization precision measurements, (2) subdiffraction molecular separation measurements, and (3) protein diffusion coefficient measurements in free solution. |
