Effects and Mechanism of Tanshinone II A in Proliferation, Apoptosis, and Migration of Human Colon Cancer Cells
| Autor: | Xue Junlai, Tongjun Liu, Xuezhu Jin, Wan Xuewen, Xiangdang Yin, Zhao Shuhua, Fang Meishan |
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| Rok vydání: | 2019 |
| Předmět: |
Programmed cell death
Down-Regulation Apoptosis 030204 cardiovascular system & hematology Flow cytometry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Western blot Cell Movement Lab/In Vitro Research Cell Line Tumor medicine Humans PI3K/AKT/mTOR pathway Cell Proliferation Arginase medicine.diagnostic_test Chemistry Cell migration General Medicine Antineoplastic Agents Phytogenic In vitro Lynch Syndrome II Sigmoid Neoplasms 030220 oncology & carcinogenesis Abietanes Colonic Neoplasms Cancer research Signal Transduction |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.914446 |
| Popis: | BACKGROUND The aim of this study was to explore the effect and mechanism of tanshinone II A on proliferation, apoptosis, and migration of human colon cancer cells. MATERIAL AND METHODS CCK-8 approach was carried out to evaluate proliferation after applying various levels of tanshinone II A to SW620 colon carcinoma cells. Flow cytometry (FC) was used to assess apoptosis. Transwell assay was performed to assess invasion in vitro, and the wound-healing assay was applied to assess migration. Western blot analysis was performed to evaluate translation of mTOR, while RT-PCR was carried out to assess transcription of VEGF. RESULTS CCK-8 assay showed that tanshinone II A inhibited SW620 proliferation in comparison to the control group subsequent to 24 h, 48 h, and 72 h (P |
| Databáze: | OpenAIRE |
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