Effects of an anesthetic mixture of medetomidine, midazolam, and butorphanol in rats—strain difference and antagonism by atipamezole
Autor: | Mayumi Takechi, Takashi Takeuchi, Kaoru Kurosaki, Yoji Saito, Yuta Kobayashi, Yumiko Kirihara |
---|---|
Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Time Factors Dose Original 040301 veterinary sciences Butorphanol Midazolam pulse oximeter Pharmacology General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley 0403 veterinary science 03 medical and health sciences medicine Animals Oximetry Rats Wistar strain difference anesthetic mixture General Veterinary business.industry Imidazoles Antagonist Atipamezole antagonist 04 agricultural and veterinary sciences General Medicine Medetomidine Anesthetics Combined Rats Inbred F344 rats 030104 developmental biology Anesthesia Anesthesia Recovery Period Anesthetic Animal Science and Zoology business medicine.drug |
Zdroj: | Experimental Animals |
ISSN: | 1881-7122 1341-1357 |
Popis: | An anesthetic mixture of medetomidine (MED), midazolam (MID), and butorphanol (BUT) has been used in laboratory animals. We previously reported that this anesthetic mixture produced closely similar anesthetic effects in BALB/c and C57BL/6J strains. We also demonstrated the efficacy of atipamezole (ATI), an antagonist of MED that produced quick recovery from anesthesia in mice. Anesthetics have various anesthetic effects among animal strains. However, the differences in the effects of anesthetic mixtures in rats are unclear. In the present study, we first examined effects of the abovementioned anesthetic mixture using three different rat strains: Wistar (WST), Sprague-Dawley (SD), and Fischer 344 (F344). Second, we examined how different dosages and optimum injection timing of ATI affected recovery from anesthesia in rats. We used the anesthetic score to measure anesthetic duration and a pulse oximeter to monitor vital signs. We found no significant differences in anesthetic duration among the three different strains. However, recovery from anesthesia in the SD strain took significantly longer than in the other strains. The antagonistic effects of ATI (0.15 mg/kg and 0.75 mg/kg) were equivalent when administered at 30 min after anesthetic mixture administration. The antagonistic effects of ATI 0.75 mg/kg were stronger than those of ATI 0.15 mg/kg at 10 min after anesthetic mixture administration. This anesthetic mixture is a useful drug that can induce similar anesthetic effects in three different strains and has an antagonist, ATI, that makes rats quickly recover from anesthesia. These results may contribute to the welfare of laboratory animals. |
Databáze: | OpenAIRE |
Externí odkaz: |